Frequently Asked Questions

Light-sheet microscopy (LSFM) is a fluorescence imaging method in which a sample is illuminated from the side with a thin sheet of light, enabling inherent optical sectioning. The fluorescence signal of the entire illuminated 2D plane is detected by a full-frame camera. Therefore, LSFM is fast, enables high temporal and 3D-spatial resolution, and restricts photobleaching/phototoxicity, striping artifacts, and other noise.

Light-sheet fluorescence microscopy can be used to image:

• 现场，固定或清除样品；
• Large samples, even of whole-body specimens;
• 快速动态生物学过程；和
• Live specimens over long periods.
  
  

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Light-sheet fluorescence microscopy optimizes detection (photon-collection) efficiency using a wide-field fluorescence microscope placed perpendicular to the light-sheet laser source. This decouples the excitation beam path from the detection beam path and provides inherent optical sectioning

一个light-sheet microscope illuminates the entire 2D focal plane at once, allowing fluorescence signal collection and full-frame camera imaging of that plane. Users can then collect high-speed 3D images with minimal sample damage, even on very large and whole-body specimens.

Watch part 1 of our on-demand webinar "布鲁克Microscopes Fill the Gap Between Traditional Fluorescence and Electron Microscopy" to see this question discussed in greater detail.

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Light-sheet microscopy is 100-1000 times faster and provides significantly gentler (less phototoxic) imaging than conventional laser point-scanning techniques like confocal or multiphoton microscopy.

Light-sheet microscopy can thereby image a wider range of samples and events on live, fixed, or cleared samples. This includes challenging large or whole-body specimens and fast dynamic biological processes that are inaccessible by other techniques.

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In fluorescence microscopy, the axial resolution is often lower than the lateral resolution. In light-sheet fluorescence microscopy, this anisotropic resolution can obstruct data analysis and cause blurriness in the axial imaging plane, especially when imaging biological structures that are smaller than the axial resolution of the microscope (e.g., membranes or subcellular structures).

一个dual view light-sheet microscope allows samples to be imaged from different angles, either sequentially, by 90° rotation of the capillary, or simultaneously. Imaging the sample from different angles with multiview fusion and deconvolution algorithms allows for isotropic spatial resolution, meaning the resolution is the same in all three dimensions, with high speed and minimal phototoxicity. Consequently, any of these methods of dual view acquisition can provide isotropic detection of rapid biological processes and exceptionally small, fine samples and target structures.

这QuVi SPIM可以配置为同时或顺序提供双视图采集，而MuVi SPIMachieves orthogonal dual view by one single sample rotation of 90°. Both microscopes thus achieve isotropic resolution and can do so even more easily when combined with our LuxControl multiview fusion and deconvolution algorithms.

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Yes, theMuVi SPIM和QuVi SPIMcan be configured to support cleared, fixed, and live sample imaging.

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这photomanipulation module can be added to every Luxendo SPIM designed for live imaging (MuVi SPIM，，，，QuVi SPIM，，，，TruLive3D Imager).

Luxendo SPIM systems are designed to provide high modularity and can be easily adapted to suit the requirements of your experiment.Contact usto discuss your specific needs with a light-sheet microscopy expert and to receive recommendations and guidance tailored to your experiment.

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由于它使用侧刷光学布置，因此，当样本中存在障碍时，灯页显微镜技术容易剥离伪像（“阴影”）。借助Luxendo Spim仪器，用户可以比以往任何时候都更快地获得无伪影的图像。

Even in the presence of inhomogeneities and absorbing obstacles,MuVi SPIM,TruLive3D Imager，，，，和LCS SPIMprevent stripe formation during image acquisition. To do so, they illuminate the sample from different angles using a pivot scanner with a beam rotation much faster than the camera exposure time. This enables these instruments to generate a homogenous, shadow-free illumination profile without compromising acquisition speed.

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Luxendo纸张microscopes have been used for multi-day continuous acquisitions on live samples lasting up to 7 days.

During these experiments, high humidity and regulated CO2 / O2 / temperature levels in the acquisition chamber ensure proper and stable growth conditions.

Luxendo纸张microscopes are compatible withLux DATA，，，，our comprehensive data processing and storage solution. This data storage and processing unit provides fast data transfer and large-capacity storage as well as remote access and high-performance processing of multidimensional image data, leveraging multi-core and GPU-based computation.

Moreover, Lux DATA is designed to manage not only the vast amount of data generated in light-sheet fluorescence microscopy, but also data from super-resolution or two-photon imaging as well.

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Luxendo的Luxcontrol软件提供了3D DATA的查看器，瓷砖缝线，多视图融合和反卷积作为图像后处理工具，可提供整个3D音量数据集，这些数据集可以用于图像分析管道。

No, all Luxendo SPIM microscopes are designed to be compact, robust, and vibration-free, and have a fully enclosed optical beam path. They can thus be positioned on any sturdy table (e.g., close to the lab bench) and operated in a daylight room. Additionally, all Luxendo instruments are certified as Laser Safety Class 1 systems, the same safety class as a laser pointer, to allow their unrestricted use.

See this question answered in more detail during the audience Q&A session at the end of the light-sheet segment of our on-demand webinar "布鲁克Microscopes Fill the Gap Between Traditional Fluorescence and Electron Microscopy" to see this question discussed in greater detail.

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这TruLive 3D和InVi SPIM Lattice Prosupport live sample imaging and can be equipped with an environmental control unit for long-term sample cultivation – even up to days of continuous acquisition. Additionally, a photomanipulation module can be added to these instruments for advanced live sample experiments.

这TrueLive 3D Imageris the ideal microscope for multi-positioning experiments (up to 100s of samples and up to six different experimental conditions in a single acquisition period) and imaging delicate live specimens such as small embryos (e.g. mouse), 3D spheroids, organoids, oocytes, and more.

这InVi Lattice Proincludes an advanced illumination module, enabling it to provide high flexibility for sample illumination using static, Gaussian, Bessel beam, or optical lattice illumination. It is thus the ideal microscope when high axial resolution and/or a large depth of field is required at high speed.

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这LCS SPIMis a dedicated microscope for cleared sample imaging and optimized for very large specimens up to entire adult cleared mice which facilitates visualization of e.g., complete neuronal or vasculature networks at cellular resolution.

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这俩MuVi SPIM和QuVi SPIMare designed to support:

• 纯活样品成像，并具有环境控制和光持持续性；
• Pure cleared sample imaging; and
• Triple-purpose live, fixed, and cleared sample imaging.

这functional flexibility of these instruments is made possible by Luxendo’s highly adaptable modular concept. Users can quickly and easily exchange the central unit, consisting of the illumination objective, detection objective, and the sample mounting chamber, to reconfigure the instrument according to the measurement requirements.

More specifically:

这QuVi SPIMis a highly versatile upright, dual view and dual color light-sheet microscope for 3D imaging of living, fixed, and cleared samples. It is optimized for long-term, high-throughput imaging at subcellular isotropic resolution.

水平4轴排列MuVi SPIM允许配备两个照明和两个检测侧，从而实现高速体积采集。因此，它对于研究大型生物活样品（例如果蝇或斑马鱼胚胎）和快速生物学过程是最佳的。与MuVi SPIM CSconfiguration, large, cleared samples up to entire mouse brains can be resolved at subcellular resolution.

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这LCS SPIMis a dedicated microscope for cleared sample imaging and optimized for very large specimens up to entire adult cleared mice. This enables visualization of structures like complete neuronal or vasculature networks at cellular resolution.

这MuVi SPIMis also optimal for studying large live samples (e.g., drosophila or zebrafish embryos) and, with theMuVi SPIM CSconfiguration, large cleared samples up to entire mouse brains can be resolved at subcellular resolution.

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