塞拉普尔®-24 Pro
高通量
Array of eight channels/three spot enables 8800 interactions in 24 hours
Highlights
针对高核应用程序量身定制
Eigenschaften
Analytical biosensor for real-time, label-free characterization of molecular interactions
连续流动SPR分析
SPR-24 PRO系统是连续的流动生物传感器，将样品和试剂作为连续流动的流提供给检测传感器。当样品或试剂注射停止时，传感器将传递“连续流缓冲区”。
The formulation of the continuous flow buffer in SPR assays can have a significant impact on the molecular interactions being measured. In many applications, measuring target interactions in a variety of buffer environments is required.
Frame Inject
The Frame Inject feature in the Sierra SPR®-24 Pro系统允许investigation of condition dependent binding / mode of action studies. The feature requires just a fraction of each buffer condition that replaces the system buffer while usage. The pre-association, as well as the dissociation occurs under the conditions, that should be investigated. After dissociation end, frame solution buffer is replaced and system buffer is flowing through the microfluidic system again. Thus experimental time and reagent costs are kept low. The same buffer can be directed to all flow cells, or up to eight different buffers can be assayed simultaneously.
通过单个针头控制最大化性能
单个针头控制
The individual needle control (INC) feature in the Sierra SPR®-24 Prosystem allows the eight sample pickup needles to be operated independently as well as in unison. Researchers have the flexibility to choose any combination of 1 – 8 needles to design their optimal assay.
单个针头控制可最大程度地提高Sierra SPR应用的性能，灵活性和范围®-24 Pro，同时还可以节省时间，材料和资源，当时吞吐量不是主要必威手机客户端要求。
八个针头探针头可以同时处理8个样品或试剂溶液。使用Inc功能，用户可以在分析过程中随时选择使用针的任何数字和/或组合。
涡流
按需吞吐量
The Sierra SPR®-24 Pro在广泛的应用中提供更快的吞吐量，从每个传感器的动力学测定到使用多个在线控件的详细分析。
该系统使用Bruker的流体动力隔离（HI）连续流微流体技术来解决流动流到24个SPRS的样品+sensor array. When integrated with an optional plate handling robot, the Sierra SPR®-24 Pro仪器每天可以测定超过4400个样品，产生超过8800个对照的减去结合响应。
塞拉普尔®-24 Procan be easily integrated into an automated scheduling software via API.
吞吐性能
灵活的Sierra SPR®-24 Prosystem is ideal for high throughput applications. It offers 24 detection spots, which are addressed by 8 injection needles. Individual needle control (INC) gives researchers the option to choose any combination of 1 to 8 needles, to optimize the performance, flexibility and scope of applications.
The 24-sensor detection array is laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independent of the others, providing maximum assay design flexibility.
多功能系统还可以节省实验室中的时间和材料，尤其是在吞吐量不是主要要求的情况下。必威手机客户端
Up to eight buffers can be used simultaneously in the instrument, using the frame inject feature. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity. The four-sensor per channel design provides maximum flexibility for control analysis, while maintaining throughput for the most complex analyses.
Enhanced productivity
Sierra Spr的设计®-24 Promaximizes control analysis flexibility, enabling high throughput for the most complex analyses. Samples can be simultaneously assayed on two controls as well as on the active sensor.
对非特异性结合与基质蛋白（如HSA或BSA）的早期分析可能至关重要。额外的在线控制不仅将所需的测定循环的数量减半，还可以通过消除多个样品制剂之间的潜在变化来提高数据质量。
从最初的测定开发到预定义的方法模板，Sierra SPRControl Software provides the perfect solution for every use case. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well-defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.
Verbrauchsmaterial
Mehr Informationen
Applications of Surface Plasmon Resonance
Tackling Challenging Targets with High-Throughput Biophysical Screening at Novartis
在此注释中，了解在这个现实生活示例中，与GenEDATA Screener一起使用SPR将SPR提升到一个新级别。
How to influence ligand density using amine coupling
Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.
筛选和表征小分子与蛋白质靶标的结合
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.
Screening and characterization of biotherapeutics
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.
使用框架注射对条件依赖性结合的研究
对条件依赖性结合的传统研究需要高的试剂成本以及冗长的运行时间。框架注入以低样本消耗以节省成本和时间。
文学室表面等离子体共振
Support
Kontakt
For Research Use Only. Not for use in clinical diagnostic procedures.