由Pasef提供动力的Timstof Pro

timstof pro

The timsTOF Pro with PASEF® technology delivers revolutionary improvements in scan speed, with enhanced specificity and high sensitivity

Omics in 4D

TIMS adds 4th dimension of separation




Accelerating 4D-Omics

使用平行的积累串行碎片(PASEF)技术> 100 Hz测序速度。
Extremely high data acquisition speed increases the throughput of biological experiments significantly.
Due to the sensitivity gains from PASEF, more peptides and their post-translational modifications (PTMs) can be identified.


由Pasef提供动力的Timstof Pro

The new standard for 4D-Proteomics™ - Dig deeper and faster into the proteome

Mass spectrometry (MS)-based proteomics has become a powerful technology for the identification and quantification of thousands of proteins. However, the coverage of complete proteomes is still very challenging due to the limited speed, sensitivity and resolution of current mass spectrometers.

TIMSTOF PRO使用平行的积累序列碎片(Pasef®)采集方法,以使用低样本量,提供极高的速度和灵敏度,以达到shot弹枪蛋白质组学和磷蛋白组学的新深度。

由Pasef提供动力的Timstof Pro


被困的离子迁移谱法(蒂姆斯)是第一位and foremost a separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. Equally as important, the TIMS device also serves to accumulate and concentrate ions of a given mass and mobility, enabling a unique increase in sensitivity and speed along with the additional dimension of separation.

Achieve near 100% duty cycle for shotgun proteomics
A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF®.

Parallel accumulation serial fragmentation (PASEF®). PASEF® strategy in a timsTOF Pro instrument where ions are accumulated while the second TIMS region is serially eluting peptide species.
由Pasef提供动力的Timstof Pro

Outstanding hardware performance

The second generation dual TIMS analyzer is optimized for shotgun proteomics needs. Due to its unique geometry ions are released dependent on their mobility from the second section of the TIMS analyzer while the further incoming ions can be accumulated in parallel in the first part of the TIMS analyzer. With the parallel accumulation technology a duty cycle of nearly 100% is achieved resulting in nearly no ion loss.

在Pro中,高级分段四极质量滤波器用于高离子传输和隔离效率。四极质量位置与TIMS分析仪的特定离子的洗脱时间同步。由于其超高的质量位置切换时间(<1 ms),因此可以为PASEF提供最佳性能®方法。

Schematic of the timsTOF Pro
由Pasef提供动力的Timstof Pro


Parallel Accumulation Serial Fragmentation (PASEF®) cycle time: By synchronizing the quadrupole with the TIMS elution time, an average of 12 precursors can be fragmented within a 100 ms time scale. Intelligent software targets low-level precursors multiple times for PASEF® MS/MS fragmentation.

考虑到速度,布鲁克专家重新设计了MS/MS技术,以满足shot弹枪蛋白质组学的要求。使用被困的离子迁移率光谱法分离肽离子,洗脱(〜100 ms)并在QTOF中检测到,生成TIMS MS热图。在Pasef中®method the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values. With the Parallel Accumulation Serial Fragmentation (PASEF®) Technology >100 Hz sequencing speed can be achieved. With the PASEF®method the MS/MS spectra quality of the low abundant peptides can be increased by selecting them several times.

PASEF®: the perfect fit for shotgun proteomics
由Pasef提供动力的timstof Pro®offers sequencing speed >100 Hz without losing sensitivity or resolution. This is achieved by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel.


由Pasef提供动力的Timstof Pro


唯一的pasef®- 可增强的质谱仪
Bruker has introduced the TIMS (Trapped Ion Mobility Spectrometry) technology in 2016 as a revolutionary ion mobility technology, achieving unprecedented levels of ion mobility resolution in an extremely compact device. The second generation of dual TIMS analyzer now enables PASEF®(Parallel Accumulation Serial Fragmentation) that is setting new standards of uncompromised speed, sensitivity and resolution for shotgun proteomics.

许多女士仪器used for shotgun proteomics require biweekly or monthly cleaning when run 24 hours a day on large sample cohorts, and performance degradation is noticeable over even shorter time periods. The superior robustness of the timsTOF Pro means that the instrument can be run 24/7 over many days or weeks without noticeable loss of sensitivity or other performance metrics.

由Pasef提供动力的Timstof Pro

PaSER Run & Done - Uncompromised data processing on the fly

The timsTOF Pro allows the analysis of hundreds of samples with minimal sample load (<200 ng) at sequencing speeds exceeding 100 Hz. All of this with uncompromised proteomic depth. This has changed the way proteomics is done, but it also unleashes a new constraint - data analysis. Modern researchers must process hundreds of samples of information dense content with the timsTOF Pro. For most pipelines this creates a bottleneck at the data analysis step. Bruker is innovating this step by introducing real-time database search capabilities called PaSER (Parallel database Search Engine in Real-time) which removes the data analysis hurdle. Using PaSER, as soon as a LC-MS run is completed, results are in hand or Run & Done.

由Pasef提供动力的Timstof Pro


Maxquant/Perseus和Peaks Studio数据处理

质量和信号强度有益于肽,蛋白质和翻译后修饰的识别和定量。Peaks Studio将从头测序与传统数据库搜索结合在一起,并经过优化用于处理TimStof原始数据。


由Pasef提供动力的Timstof Pro


使用100 ng的HeLa Digest,具有短梯度(30分钟,60分钟和90分钟)的深蛋白质组覆盖率(30分钟,60分钟和90分钟)
Speed and sensitivity translating into high proteome coverage
The combined power of trapped ion mobility spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF®) allows for greater proteome or sub-proteome (e.g. phosphoproteome) coverage without requiring the large sample amounts (typically 1-4 µg of a peptide mixture on column) often used in proteomics.
Superior results can be obtained from less than 200 ng sample load, therefore reducing both sample preparation costs and MS maintenance frequency. Using a 90 min gradient length more than 5400 protein groups can be identified from a typical human cell line lysate. The timsTOF Pro can also deliver reproducible quantitative information from smaller amounts of highly complex mixtures.

由Pasef提供动力的Timstof Pro

Latest timsTOF Pro Application Notes

实现高通量,高灵敏度的靶向蛋白质组学。PRM-PASEF®已经开发了采集方法来翻译并行积累序列碎片的优势(Pasef®) acquisition strategy to the targeted proteomics field.

The combination of integrated ion mobility with warp-speed MS/MS acquisition on the timsTOF Pro paves the way for deep, molecular level understanding of β-cell physiology and potential new targetable pathways for diabetes.

高通量4D-Proteomics™ – Application of dia-PASEF®and the Evosep One for short gradients
The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and sensitivity and Parallel Accumulation Serial Fragmentation (PASEF®)



“我们现在知道,在二维分析时(保留时间和m/z)时,肽混合物仍然非常复杂。在原则上,添加一个维度应该为我们带来很长的前进。除了分离的附加维度,timstof Pro为我们提供了极高的速度和灵敏度,以更深入蛋白质组并使用较少的样品材料。”

Prof. Dr. Matthias Mann, Director Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Germany




timstof Pro相关信息