NMR Methods for Labeled Proteins

Topics:

• Overview of NMR experiments for protein structure determination.
• Introduction to triple-resonance experiments.
• Bruker software for biomolecular applications.
• Set-up and acquisition of triple-resonance experiments.
• 介绍了脉冲。
• Recent methodological developments in bio-NMR

Remarks:

This is a course for experienced users of Avance spectrometers working with labelled protein samples. The background for choosing and setting-up 3D-triple-resonance experiments for proteins is given. During the practical sessions representative spectra such as 3D-¹⁵N-NOESY-HSQC, HNHA, HNCA, HNCOCA, CBCA(CO)NH and HCCH-TOCSY are acquired. The implementation of the shaped pulses needed for biomolecular NMR and the use of adiabatic pulses, is covered.

Prerequisite Qualification:

Participation in the courseAdvanced NMR Methodsor corresponding knowledge.

Dates:

October 7^th- 8^th, 2021

Location: