Light-Sheet Microscopes

MuVi SPIM Family

您的多样化应用

MuVi SPIM Products

最终的灵活性和性能

The MuVi SPIM is a very versatile and flexible setup. Its horizontal 4-axis arrangement of having two illumination and two detection sides enables high-speed volumetric acquisition of the data without the need of sample rotation. This can be the key factor in increasing temporal resolution.


Despite the fact that sample rotation is not necessarily needed for a non-isotropic acquisition, the MuVi SPIM provides this degree of freedom, such that isotropic resolution can be achieved.

LoMásDestacado

Highlights

Multiview Imaging Offers Configurable Set Up

The MuVi SPIM Family consists of a basic common concept: the horizontal 4-axis setup with the possibility of sample rotation. It is a modular design that allows for a tailored configuration, and ensures upgradeability. Looking at it in terms of categories, one could distinguish live imaging, cleared imaging, and photomanipulation, but all of these categories are within the common concept of multiview acquisition.

各向同性分辨率具有360°照明和检测

The MuVi SPIM’s horizontal setup is designed to image large volumes very fast. The unique 4-axis concept with its two-sided illumination beam paths and two-sided detection paths allows four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation, avoiding shadowing effects and facilitating long-term imaging.

Flexible Multi-User Design

The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that holds all the objective lenses. The octagon can be easily removed and replaced while maintaining its optical alignment. This makes the MuVi SPIM very flexible and user-friendly, a big advantage especially in multi-user environments.

Robust Design

The MuVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and through short optical paths and thermally rigid case design. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning.

Características

3D Imaging of Cleared Samples

Cleared Sample Module

The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that can be easily removed and replaced while maintaining its optical alignment. This makes the MuVi SPIM very flexible and user-friendly, a big advantage especially in multi-user environments.

The cleared sample octagon and an overhead sample positioning unit installed on the microscope’s top plate can transform a MuVi LS system into a MuVi CS system.

The octagon can be easily removed from the microscope for maintenance and cleaning of the sample chamber and the objective lenses that have been exposed to the mounting solution. The sample chamber is compatible with a broad range of solutions used in different clearing protocols. The sample chamber can accommodate large samples such as mouse brains and other complete organs. Sample mounting from above enables easy sample access and optimized travel range suited for various mounting methods (e.g. hook, plate, pin or cuvette).

Software

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。

Electronics, Microscope Software and Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • 用于交互式显微镜控制和实验设计的灵活GUI
  • Computer with 256 GB RAM, Intel dual 8 core CPU
  • 用于数据流的高速RAID控制器,RAID O中的8×4 TB本地存储
  • GeForce RTX 3070 graphics card
  • 2 x 10 gbit/s,2 x 1 gbit/s,可选2 x光端口
  • 43 inch 4k UHD display

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