MuVi SPIM PM
Lo más destacado
MuVi SPIM PM with Photomanipulation
Whether you want to cut structures within cells and tissues (photoablation), photobleach fluorescence in selected regions of interest for FRAP or activate proteins for optogenetics, the MuVi SPIM PM module can be configured to meet your needs by choosing between three different types of lasers systems. It can be added to both MuVi SPIM LS and CS (photomanipulation only possible when LS octagon mounted).
Características
照明
The MuVi SPIM PM illumination beam is coupled into the right-side detection objective lens, creating a diffraction-limited illumination spot. Its size is determined by the properties of the detection objective lens.
A 2D scanner allows to freely position the illumination spot in 3D in the sample while it is being imaged – fully integrated in the acquisition software. Complex illumination regions (point, straight and freeform line, square) are either part of the experimental workflow or can be defined interactively on the fly while a 3D imaging experiment is carried out.
For different applications, the MuVi SPIM PM can be configured with different types of lasers, ranging from CW UV to pulsed IR lasers.
Muvi Spim PM设计
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Aplicaciones
光掌握应用
Microglia Response to Axonal Damage
The axon of a neuron in the zebrafish brain was selectively dissected by means of IR laser ablation (MuVi SPIM). Thirty minutes after ablation, four microglia reached the damaged axon.
Image taken from:
de Medeiros, G., Kromm, D., Balazs, B. et al. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep 10, 1942(2020).https://doi.org/10.1038/s41598-019-54349-x
Especificaciones
Specifications
| Detection Objective | Adressable FOV | |
| Olympus 20x / 1.0 NA | 600 µm | |
| Nikon 16x / 0.8 NA | 830 µm |
照明Optics
- Chromatic correction from 400 to 700 nm (UV/VIS) or from 780 to 1100 nm (IR)
- Flexible ROI generation by beam scanning, available ROIs: point, straight and freeform line, square
- Full software control
- Diffraction-limited spot
- Coupled into the right detection objective lens
Available lasers
- 脉冲IR激光1030–1040 nm，200 fs脉冲长度，1.5 w，用于光照
- Up to two CW diode lasers, between 405 and 685 nm, up to 100 mW for photoactivation, photobleaching, optogenetics
- CW or pulsed laser 780 nm, 100 fs pulse length, up to 500 mW, for photoactivation, optogenetics
