被困的离子迁移率光谱法（TIMS）

离子迁移率光谱法（IMS）是一种强大的分析技术，在过去五十年中已广泛应用，主要用于化学物理和分析化学应用。直到最近，就探索了IMS与MS（IMS-MS）的潜力，以进行肽和蛋白质的分离，鉴定和定量。

被困的离子迁移率光谱法（TIMS）is an IMS technique where ions are propelled through the TIMS tunnel by a gas flow. An electrical field controls each ion from moving beyond a position defined by the ion’s mobility, where the push it experiences from the gas flow matches the force of the electrical field. Ramping down the electrical field allows selectively releasing ions from the TIMS tunnel according to their mobility.

By incorporating a TIMS device at the front of a quadrupole time-of-flight (QTOF) mass spectrometer, ions can be accumulated for a specific amount of time before being released for MS analysis. Using the PASEF® method, peptide ions are separated using TIMS, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map. In the PASEF® method, the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values.

蒂姆斯
被困的离子迁移率光谱法（TIMS）is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. Equally as important, the TIMS device also serves to accumulate and concentrate ions of a given mass and mobility, enabling a unique increase in sensitivity and speed along with the additional dimension of separation.

A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF®.

PASEF
With speed in mind, Bruker experts redesigned MS/MS technology to meet the requirements of shotgun proteomics. With PASEF® technology >100 Hz sequencing speed can be achieved, and the MS/MS spectra quality of low abundant peptides can be increased by selecting them several times.

Bruker的TimStof Solutions加速了多样化的研究应用程序，提供了信心和清晰度，以识别和量化具有挑战性的化合物。