NMR Instruments

Ascend GHz Class

Ultra-high field NMR is one of the only analytical technologies that allow for advanced research on structural functional biology of proteins and protein complexes including intrinsically disordered proteins (IDPs). The Bruker line of 1.1 and 1.2 GHz NMR instruments provide unsurpassed stability and spectral resolution allowing researchers to see further into the world of functional structural biology than ever before.

先进的研究

具有高级NMR技术

Ascend-1.2-Ghz-Freigestellt

罗马斯卡多多

强调

1.2 GHz
布鲁克’s novel UHF NMR magnet technology makes it possible to achieve magnetic flux densities of 28.2 Tesla, which corresponds to a proton resonance frequency of 1.2 GHz
Hybrid
力量的1.1和1.2 GHz核磁共振磁体利用11月el hybrid design with advanced high-temperature superconductor (HTS) in the inner sections and low-temperature superconductor (LTS) in the outer sections of the magnet.
高分辨率
布鲁克’s 1.1 and 1.2 GHz spectrometers have been optimized for high resolution NMR experiments. The exquisite field homogeneity and temporal field stability surpass other high field magnets, e.g. driven mode systems.

For many years, high-resolution NMR was limited to a magnetic field of 23.5 Tesla, equivalent to a proton resonance frequency of 1.0 GHz. This limit was set by the physical properties of metallic, low-temperature superconductors (LTS), and it was first reached in 2009 with an Avance® 1000 spectrometer at the Ultra-High Field NMR Center in Lyon, France.

高温超导体(HTS),首次在20世纪80年代发现,在低温下向甚至更高的磁场打开门,但在YBCO HTS胶带制造和超导磁铁技术方面的挑战进一步进一步进展令人生意。

Bruker独特的1.1和1.2 GHz NMR磁铁使用新型的混合动力设计,内部部分和磁体外部的高温超导体(LTS)中的高温超导体(HTS)。上升1.1和1.2 GHz是稳定的,标准孔(54毫米)磁铁,具有精致的均匀性和现场稳定性,与高分辨率NMR的要求符合要求。1.2GHz光谱仪可提供不同的超高场探针,包括用于溶液状态NMR的冷冻毛皮,以快速纺丝MAS固态NMR探针。

Caption: Artist’s impression of a UHF NMR magnet. The solenoid magnet consists of several concentrically arranged magnet sections made from different superconducting materials. NbTi (yellow) is used in the outermost sections of the magnet, Nb3Sn (red) in the mid-field region, and high-temperature superconductors (blue) in the central section. Cryogenic shim coils are used to improve the homogeneity of the magnetic field. Operation with a persistent ensures that the magnetic field is very stable over time

Bruker GHz-Class NMR用于功能性结构生物学研究

Bruker通过先进的NMR解决方案帮助揭示功能 - 结构生物学研究。新型GHz级NMR技术使高级研究能够进入蛋白质 - 配体相互作用的亲和力和特异性的结构基础,包括更好地了解细胞膜蛋白的结构特征,以及蛋白质折叠和聚集的分子机制。

1.2GHz NMR的频谱分辨率和敏感性增加已经使研究团队能够在蛋白质中更深入地看,更好地了解淀粉样蛋白聚集的初始步骤以及TAU蛋白的功能和结构,通常与之相关Alzheimer’s disease.

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2019年,Bruker成功安装了田纳西州孟菲斯的圣乔德儿童研究院的世界上第一个1.1 GHz NMR系统。

圣裘德的儿童研究院的结构生物学系主席查尔马拉姆斯·卡洛摩摩博士:“我们兴高采烈地收到了第一个1.1 GHz NMR光谱仪,这将是我们在动态分子领域进行研究的最重要工具分子伴侣和蛋白激酶等机器。我们赞扬了Bruker对这个令人印象深刻的技术成就。“

Shortly after, in early 2020, Bruker installed the world's first 1.2 GHz NMR system at the CERM of the University of Florence. CERM is an Italian center of the European research infrastructure in structural biology.

在成功安装之后,Lucia Banci教授和Claudio Luchinat在佛罗伦萨大学的Cerm,表示:“我们很激动到我们的实验室成功安装了世界上第一个1.2 GHz NMR光谱仪。我们期待着将仪器在我们对与神经变性疾病相关的蛋白质的结构和功能的研究中使用,例如阿尔茨海默氏症和帕金森氏病,以及癌症和病毒蛋白质结构和功能研究。现在,我们正在积极研究SARS-COV-2蛋白,我们将很快从这个冠状病毒中记录蛋白质的第一个1.2 GHz NMR光谱!“

Later in 2020, Bruker successfully installed the world’s second 1.2 GHz NMR spectrometer at Eidgenössische Technische Hochschule (ETH) Zürich in Switzerland. This 1.2 GHz spectrometer is the first one that is configured for solid-state NMR.

At the time, Professors Beat Meier, Matthias Ernst and Alexander Barnes at ETH stated: "We are very excited to have the world's first 1.2 GHz solid-state NMR spectrometer successfully installed in our lab. The system was delivered just a couple of months ago and the installation and energizing of the NMR magnet went exceptionally well. The completion of the installation marks the culmination of a project that we started with Bruker almost a decade ago. We are very much looking forward to starting our first ultra-high field solid-state NMR experiments.“

ETH utilize their 1.2 GHz NMR system to enable the development of new solid-state NMR techniques, and to apply these techniques to study materials and biological systems, including proteins fibrils which are linked to diseases such as Parkinson's and Alzheimer's. The 1.2 GHz spectrometer will also be used as a basis for further improving NMR methodology towards in-cell structural biology, and to investigate solid catalysts and functional materials, e. g. for energy conversion and data storage.

在2021年初,布鲁克很自豪地宣布成功地安装其第四个1.2 GHz NMR系统Max Planck Institute (MPI)for Biophysical Chemistry in Göttingen, enabling their research teams to deliver new insights into the SARS-CoV-2 nucleocapsid (N) protein, and aiding the deeper molecular understanding of Parkinson’s and Alzheimer’s diseases.

Professor Christian Griesinger, Director and Scientific Member at the Max Planck Institute for Biophysical Chemistry in Goettingen, commented: “The new 1.2 GHz spectrometer will allow us to characterize droplets and oligomers of IDPs that are key markers in diseases such as COVID-19, neurodegenerative diseases and cancer, and which cannot be studied using crystallography or cryo-EM.”

Markus Zweckstetter博士,Goettingen大学教授和德国神经退行性疾病中心的集团领导者,补充说:“我们在安装新的超高场NMR系统后的第一个实验集中在SARS-COV-2核衣壳上N-蛋白质是病毒宿主相互作用和病毒复制生物学的关键相关性。病毒复制机械的液体状特性与N-蛋白的许多本质无序区域组合使得这项研究非常适合GHZ-Class NMR。“

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