我nVi SPIM Lattice Pro
伪造的侯爵夫人
我nVi SPIM Lattice Pro
The InVi SPIM Lattice Pro provides the highest level of flexibility for illuminating samples using a proprietary Advanced Illumination Module (AIM). It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.
Caractéristiques
我llumination and Detection
The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices.
The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs.
Invi Spim晶格专业设计
Not available in Germany.
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Applications
Live Sample Applications
从下面的客户浏览一些应用程序数据。研究人员以多种方式使用Invi Spim晶格Pro，包括胚胎发生和发育生物学，器官，细胞培养物，神经生物学和神经发育，植物等。
Mitosis in HeLa Cells
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
我maged on the InVi SPIM Lattice Pro.
Visualization: Imaris (Bitplane).
Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
3D Imaging of a Spheroid
带有EGFP和MRFP标记的球体在Invi Spim Lattice Pro上成像。测试了每个标签的三种照明图案：高斯梁，贝塞尔束和光学晶格。光学晶格为EGFP标记提供了最佳的结果，而高斯束最适合MRFP标记。
Courtesy of:
马丁·斯托克尔
University of Konstanz
德国
HeLa Cell Culture
HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.
Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands
规格
Specifications
| 我llumination | Detection |
Effective Magnification |
Field of View | 像素是ze | Optical Resolution |
| 28.6x / 0.7 NA | 尼康25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
我llumination Optics
- 从440到660 nm的色度校正
- Light-sheet generation with static illumination or by beam scanning
- Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
- Special Optics 28.6x 0.7 NA water immersion objective lens
Detection Optics
- 尼康CFI APO 25X W 1.1 NA浸入物镜镜头
- 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- 峰量效率（QE）：82％ @ 560 nm
