Microfluidics Unit
Caractéristiques
在定位显微镜中，应用程序正在发展，其中成像的目标数量远远超过了可以分离的探针的数量。使用微流体的顺序标记可以解决更多的目标。
The Bruker microfluidics accessory is a unit designed to allow sequential labelling to be incorporated into localization microscopy protocols with theVutara VXLsingle-molecule localization microscope. The unit is controlled by Vutara'sSRX softwareand allows flexible configuration of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unlimited number of probes and image them in a sequential fashion.
信息学
The probe associated with each sequence has a specific color that is user-defined, and that is used as a color scale for visualization of experiments. During analysis with SRX, individual probes can be analyzed, or analysis can be carried out that compares probes.
Applications
Applications
Bruker微流体单元可与Vutara一起使用，以解决需要成像大量目标的任何定位显微镜应用。提供了15个探针小瓶，该系统允许在实验过程中互换探针小瓶，从而允许无限数量的目标。
Example localization microscopy applications:
- OligoSTORM
- DNA PAINT
- Multiplexed antibody labelling
- 刷新风暴
The Bruker microfluidics unit is also compatible with theOpterraconfocal for serial labelling confocal applications.
规格
Specifications
| Dimensions | 370mm x 300mm x 204mm |
| 水库 | 15 2.0 ml Eppendorf tubes for probes |
| 清洗/缓冲液库 | Two 15 ml, one 50 ml, one 100 ml |
| Flow rates | 1 µL/min – 1000 µL/min |
| Pumping method | Air pressure (self-contained pump) |
| Interchangeability | 水库can be changed out while system is in use for an experiment; allows an unlimited number of probes to be used. |
| Dead volume: Fluidics unit: | 85 µL用于探针通道，洗涤/缓冲通道为190 µL |
| Tubing from fluidics unit to flow chamber | 2 µL/cm |
| Flow cell | 标准流动池150 µL |
逻辑
Software
Control of the unit is via SRX software. The software has a flexible interface module (Fluidics Manager) for the set-up of fluidics. An unlimited number of sequences can be set up, each with its own unique steps.
