Super-Resolution Microscopes

Vutara VXL

比单分子定位microscope (SMLM). A benchtop multimodal bioimaging workstation designed for your lab.
Vutara VXL

Faits marquants

Vutara VXL

The Vutara VXL comprehensive biological workstation for nanoscale biological imaging opens an affordable and easy-to-use path for both core facilities and individual investigators to enter the world of super-resolution imaging by incorporating Bruker’s industry-leading single-molecule localization microscopy (SMLM) technology in a streamlined system with compact footprint. The new system enables research on DNA, RNA and proteins, from macromolecular complexes and super-structures, to chromatin structure and chromosomal substructures, to studying functional relationships in genomes and in various subcellular organelles. This novel system also supports advanced spatial biology research in extracellular matrix structures, extracellular vesicles (EV), virology, neuroscience, and live-cell imaging. When combined with Bruker’s unique microscope fluidics unit, Vutara VXL enables multiplexed imaging for targeted, sub-micrometer multiomics in genomics, transcriptomics, and proteomics research.

最深
3D imaging
使用专有双翼飞机技术实现>30μm成像的深度。
无缝的
多模式成像
Combines with robust, flexible software for studies across length scales.
融合的
microfluidics
Provides easy yet unlimited multiplexed imaging for spatial genomics, transcriptomics, and proteomics.

Caractéristiques

特征

每次收购都是3D

Proprietary biplane technology, combined with a spatial filter in the emission light path, allows you to acquire 3D data with every acquisition. For thicker specimens, the Vutara allows you to easily perform a Z series and automatically localizes and reconstructs the entire volume.

Single-Molecule Imaging Beyond the Cover Slip

The Vutara VXL is capable of imaging far from the surface of the coverslip to accommodate a wide range of sample types. Thanks to the proprietary biplane technology, the Vutara VXL with the SRX software can perform single-molecule localization microscopy on more sample types than any other commercial single-molecule localization microscope available. Making cultured cells, cell colonies, tissue sections, and entire model organisms accessible for your single-molecule localization experiments.

单分子样品类型。

将本地化变成信息

Vutara的定量定位显微镜套件使您可以将本地化变成有意义的结果。Vutara的SRX WorkFlow驱动软件可指导用户进行超分辨率单分子定位实验的设置,校准,成像,处理和分析。SRX软件将实时本地化处理与强大的3D可视化和分析工具相结合,以使研究人员快速创建出版物质量视频,图像和测量。

申请

规格

规格

成像方式
超分辨率定位显微镜(SMLM)
  • SMLM具有高Z分辨率用于风暴,手掌,油漆和相关的超分辨率应用
  • 具有大型FOV的定位显微镜,用于超分辨率多路复用基因组应用的优化
广场显微镜
  • 大型FOV的荧光显微镜
  • Transmitted light microscopy with large FOV
Imaging Optics
Excitation lasers (nominal laser power at diode)
  • 405 nm, 120 mW
  • 488 nm,2000兆瓦(可选)
  • 555 nm,2000兆瓦
  • 638 nm,1200兆瓦
  • 750 nm,1500兆瓦(可选)
平坦的照明
  • 平方帽照明从方形光纤保证的平坦激发曲线
Multi-color acquisition
  • Up to 5 colors sequential
Multi-plane imaging
  • 同时对两个焦平面的成像允许1 µm深度区分(Z-stack模式下可能更大的Z范围)

相机

  • Orca Flash 4.0 V3 SCMOS摄像机
  • Orca Fusion BT SCMOS摄像头(可选)
客观的
  • 60x magnification
  • 1.3 numerical aperture (NA)
  • 硅油浸入
  • 0.3 mm working distance
  • #1.5H cover glass (0.170 ±0.005 mm)
视野(FOV)
  • 200 µm x 200 µm多路复用定位显微镜和广场成像
  • 使用开关(风暴,棕榈,油漆)和3D定位(双翼飞机检测)的SMLM 50 µm x 50 µm
  • Larger FOV with tile scanning
Single Molecule Localization (SMLM)
3D SMLM方法
  • 拟合测得的PSF以最大化XYZ的精度
  • Proprietary biplane for high accuracy z-resolution and deep sample imaging
SMLM分辨率
  • 横向20 nm(xy)
  • 用双翼飞机轴向(z)50 nm
成像深度
  • > 30 µm (typical, dependent on sample)
舞台,聚焦驱动器和样品支架
xy-stage
  • SMLM具有高Z分辨率用于风暴,手掌,油漆和相关的超分辨率应用
  • 具有大型FOV的定位显微镜,用于超分辨率多路复用基因组应用的优化
z-focus
  • 课程重点以本地化样本
  • 快速Z-stack获取的良好重点
漂移校正
  • 数据获取期间的主动焦点漂移校正
  • 焦点漂移<30 nm 10分钟
  • xy drift correction post-acquisition
环境控制
  • Live cell incubation with humidity, CO2, and temperature control (optional)
多路复用(可选)
微流体单元用于定位显微镜应用中的顺序标记
  • 样品缓冲液的多路复用交换(可选流体单元)
  • 15 x 2 ml reservoirs for imaging probes
  • 2 x 15 ml, 1 x 50 ml, 1 x 100 ml for washing and buffer solutions
  • 1 µL/min - 1000 µL/min流速
  • 无缝的integrations in SRX microscope control software
维度和环境
紧凑型桌面设计
  • 如果显微镜和激光发射安装在同一表上,则需要空间:120 cm x 75 cm(4英尺x 2.5英尺)
  • 没有相机的显微镜:

34 cm x 40 cm x 34 cm, (1.2 ft x 1.3 ft x 1.2 ft)

35 kg (78 lbs)

  • Laser launch:

33厘米x 53厘米x 32厘米(1.0 ft x 1.7 ft x 1.0 ft)

30公斤(65磅)

  • 电子盒堆叠在激光发布的顶部:

53 cm x 35 cm x 14 cm (1.7 ft x 1.1 ft x 0.4 ft)

8公斤(17磅)

在典型的实验室环境中运行
  • The light-tight design does not require dimmed room light
Vibration insulation included
  • The system includes vibration insulation (sturdy table or bench required for installation)
  • 可选:客户可以为具有挑战性的环境提供光学表
软件
Workflow oriented software for easy data acquisition
  • SMLM模式

STORM / dSTORM

棕榈

PAINT

其他基于眨眼的方式

  • 基因组学方式

Chromatin tracing

Smfish(在开发中)

  • Multiplexing workflow

跟踪探针周期和位置

支持无限数量的探针

  • 校准工作流程以确保卓越的定位精度
SMLM处理
  • 基于测量点传播函数(PSF)的XY拟合
  • 由校准双翼数据确定的Z位置
  • 数据获取期间的初步本地化
  • 验证本地化的统计工具
粒子跟踪
  • Diffusion analysis based on nearest neighbors
  • 基于稀疏发射极跟踪的粒子分配
  • Mean-square displacement and distribution analysis and plots
  • Mean velocity and angle calculations and plots
Fluidics Manager
  • 设置和控制多路复用成像工作流程
  • 跟踪探针标签和元数据
Chromatin tracing (optional)
  • 染色质体系结构的光学重建

漂移校正

  • 自动相关
  • 嵌入样品中的基金会
统计分析工具
  • Spatial distribution

里普利的K功能

Pair correlation

最近的邻居

  • 聚类分析

dbscan

光学

德劳尼

Particle counts

Volume and density calculations

回旋和球形半径的确定

主成分分析(PCA)

质心的计算

  • 共定位

Pearson’s correlation coefficient

曼德的重叠系数

强度相关商

交叉对相关

关节直方图

STORM-RLA

  • 分析分析

傅立叶环相关

标签密度分辨率分析

本地分辨率相关映射

最近的邻居分析

融合的visualization
  • 2D和3D可视化
  • 点云和点裂
  • Volumetric slices
  • 等法表
  • Maximum intensity rendering
  • Depth color coding
  • 时间框架颜色编码
  • 通过探测颜色编码
  • Cluster hull visualization
  • 簇的线框可视化
  • 轨道轨迹的轨迹
Open data formats
  • Raw images are stored in highly efficient binary format and can be exported in ome-tiff format
  • 漂移校正数据的导出
  • 本地化以高效的二进制格式存储,可以导出为.csv或matlab二进制文件
  • Results from cluster analysis are stored and can be exported
  • Export of results from statistical analysis as .csv or MATLAB binary files
  • All imaging and processing settings are stored in xml or Jason format
  • 图像可以作为矢量图形或用户定义的大小/DPI导出
  • 规格for all data formats are published
Additional Data Storage
网络附加存储(NAS)单元(可选)
  • 90 TB有效存储
  • 2 GB/秒转移率

逻辑

A Complete Solution, From Acquisition to Analysis

借助SRX软件及其定量定位显微镜分析套件,Vutara VXL可以从生物样品中提供视觉和定量信息。通过定位单个分子,Vutara可以生成惊人的3D图像,同时提供深入的定量分析工具。

SRX software.

Webinaires

Témoignages

在设计学术界和行业的显微镜方面有超过15年的经验,我很自豪地为Vutara超分辨率显微镜的发展做出贡献。通过Biplane 3D检测和快速SCMOS成像,Vutara具有市场上最先进的超分辨率显微镜。

Joerg Bewersdorf, Ph.D., Yale University

在扫描了市场上的超分辨率显微镜以及使用我们自己的样品进行个人访问和测试的大多数市售系统之后,我可以说我对Vutara的SR-350印象深刻。特别是,他们的成像软件的用户友好性以及超分辨率模式下的3D功能给我留下了深刻的印象。

Vutara has an excellent support team and staff as a whole making our transition to super-resolution a well-supported experience. If you're looking to advance your research by super resolution microscopy, I can confidently say that Vutara's systems are an excellent choice.

麦吉尔大学核心设施主任托马斯·斯特罗(Thomas Stroh)博士

在比较电子显微镜时,特别是考虑样品制备时,您可以在超分辨率系统上获取的数据量令人难以置信。

Peter McPherson, Ph.D., McGill University

Having published with a Vutara super-resolution system, I can confidently say that they offer one of the most advanced super-resolution microscopes available. Their attention to detail and ongoing close collaboration makes them one of my preferred microscopy vendors.

波士顿大学Brigitte Ritter博士

续专家

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