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原子力显微镜

Dimension FastScan Bio

快速扫描大型样品

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Dimension FastScan Bio

维FastScan生物原子力显微镜(AFM) enables high-resolution research of biological dynamics, with temporal resolution of up to 3 frames-per-second for live sample observations. What’s more, it does this while making the AFM easier to use than ever before. Built upon the world’s most advanced large-sample AFM platform, the FastScan Bio AFM adds specialized life science features to this platform for high-resolution, live-sample observation of interacting molecules, membrane proteins, DNA protein binding, inter-cellular signaling and many other dynamic biological studies.

即时的
panning, zooming and scanning
允许研究人员观察并研究生物分子机制如何发挥作用。
Simplified
sample engaging and controls
Deliver easy and immediate nanoscale bioimaging.
Smart Engage
algorithms
Provide flexibility for commercially available or custom-made probes.
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Caractéristiques

Features

High-Resolution Biological Dynamics

In-situ high-resolution dynamic studies with nanoscale resolution are a reality for a rapidly evolving branch of biological research. Dimension FastScan Bio breaks longstanding barriers to provide these capabilities for routine experimentation. These breakthroughs allow many more researchers to observe and study biomolecular structures and mechanisms. FastScan Bio’s high-resolution and high-speed scanning provide the best available bio tool for the observation of molecules, proteins, DNA, RNA, living cell membranes and tissues, and many other dynamics studies.

（1：1）DOPC/DPPC的时间序列支持平面脂质双层。连续的图像记录为每秒0.16帧，证明了较高的DPPC凝胶相结构域的稳定性，因此在高扫描速度下快速扫描的高度敏感力控制。在水性条件下使用TappingMode™进行成像。显示了高度图像。（图像尺寸=3μm; z-scale = 3.2nm。）

Technology Designed for Live Samples

FastScan Bio's innovative AFM technology enables high-speed scanning and a seamless user interface to render immediate panning, zooming and continuous tracking of samples in fluid. Bruker’s new innovative probe design with unique cantilever shape and coating enables an uprecedented combination of imaging speed and softness. The resulting system delivers the scanning speed required for high-resolution spatiotemporal studies with the greatest simplicity ever seen in a commercial AFM system.

  • Simplified sample engaging and controls for immediate imaging
  • 实时平移，缩放和扫描
  • Feature tracking and Movie creation tool
  • Micro-volume fluid cell with controlled fluid exchange

Applications

Applications

FastScan Bio Data Gallery

每秒0.5帧的DNA的高分辨率图像。Lambda Digest DNA在云母表面吸附，并在水性条件下使用Tappingmode成像。插图图显示在每秒3帧时捕获的相同的DNA链。（图像尺寸= 500nm;特征高度= 1.8nm; z-Scale = 4.3nm。）。
CM15活性大肠杆菌细胞上的CM15抗菌活性的时间序列。将CM15（10μg/mL）添加到T = 0s的成像流体中，并以1024 x 256像素的分辨率记录每18s的图像。一旦首先在〜7.5分钟观察到CM15的效果，就会显示时间序列中的每个第七张图像。HS-AFM成像显示细菌细胞表面的粗糙度或波纹增加，这被认为是将CM15掺入外膜的直接结果。观察到这些变化的开始是在不同时间的单个细胞发生。
FastScan AFM图像允许直接的观察nanoscale ordered structures, corresponding to closely packed porin molecules, on the native outer membrane of live E. coli cells. CM15 (20μg/mL) was added to the imaging fluid at t=0s and images were recorded every 8 seconds at a resolution of 1024 x 256 pixels. Every second image in the time series is shown after ~96s incubation when the effects of CM15 were first observed as an increase in membrane roughness (corresponding graph shown above), accompanied by the disappearance of the ordered porin structures, membrane micellization, and the appearance of “pore-like” lesions in the membrane surface.
AFM image sequence of the leading front of a migrating stem cell showing the formation of two extended lamellipodia. As the cell ‘crawls’ forward, membrane flows to the cell front filling in the space between the lamellipodia. Images were recorded every 40 seconds at a resolution of 512 x 128 pixels.
Graph showing the rapid increase in surface roughness of the outer membrane of a live E. coli cell after exposure to the antimicrobial peptide CM15 (20μg/mL). Data points corresponding to the individual images in video strip below are indicated by the arrows. The inset phase images show the location on the cell where the high-resolution data was obtained (red boxes) and confirm that the observed changes to the outer membrane structure occurred over the entire cell and thus were not tip-induced.

Accessoires

Accessories

Bruker AFM Probes

Bruker AFM Probes

Find a wide variety of AFM probes, cantilevers and accessories in our online store. Our AFM expertise is built into every probe!
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Testimonials

Hear What Our Customers Have to Say

FastScan Bio AFM在常规AFM上的性能提高了，因为我们已经达到了构成折纸纳米动物的DNA链的螺旋重复的分辨率。该AFM平台的较小快速探针和高稳定性是在液体下获得高分辨率成像的关键。这种改进的分辨率正在帮助我们回答有关未来纳米材料应用DNA折纸的形成和稳定性的问题。必威手机客户端

英国利兹大学阿斯特伯里结构分子生物学中心尼尔·汤姆森教授

FastScan Bio的较高速度和稳定性使我们对膜纳米域动力学的首次直接见解。在升高温度下，我们捕获了模型细胞膜运动中运动的电影，使我们量化了域线张力，并观察到大型脂质结构域的分解为较小的纳米级波动。现在，我们可以将域的寿命与临界温度达到和高于临界温度的尺寸直接相关联。

Dr. Simon Connell, School of Physics and Astronomy, University of Leeds, UK

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