- HUPO 2020 TIMS技术商业化科学技术奖
- 真正的单细胞1,000蛋白质组来自Mann Lab Leverage Dia-Pasef®and further improvements in ultra-low flow Evosep chromatography and modified TIMS/PASEF set-up
- significant Improvements in Quantitative Targeted 4D-Proteomics with prm-PASEF®
- 许可PhoXcross-linker from Heck Lab enables caps-PASEF structural proteomics
BILLERICA, Mass.--(BUSINESS WIRE)-- At the virtual 19th人类蛋白质组组织世界大会（hupo2020.org），布鲁克公司(Nasdaq: BRKR) today announced that Melvin A. Park and Oliver Raether were awarded theHUPO科学技术奖for the commercialization of Trapped Ion Mobility Spectrometry (TIMS) and the Parallel Accumulation Serial Fragmentation (PASEF®） 方法。该奖项认可了改变科学家进行蛋白质组学方式的创新，从而验证了Timstof Pro在为大型队列深度4D蛋白质组学转化研究中提供短梯度方面的作用。布鲁克还借此机会认识了Pasef共同发明的贡献Professor Matthias Mann。
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Figure 1: Real-time PaSER can monitor 4D-Proteomics data acquisition (Photo: Business Wire)
A. Real-Time Database searching withPaser™ for “Run and Done’ 4D-Proteomics
Bruker further announced the release ofPaser，一个完整的基于GPU的设备，在“实时”中启用蛋白质组学数据库搜索，以最近宣布的收购为基础IP2software. The term ‘PaSER’ was coined by约翰·耶茨三世教授andDr. Robin Parkfrom The Scripps Research Institute and stands forPArallel Databasesearchengine inrEAL时间。独特Paser建筑使用并行,多线程earch engine running on GPUs to search proteomics results in real time at or faster than the data acquisition. This result is ‘Run and Done’ high-throughput 4D-Proteomics where scientists have identified peptides and protein groups as soon as the experiment is complete.
Professor Yates will present on “The Synergies of Mass Spectrometry and Informatics” in Bruker’s Virtual Industry Seminar (hyperlinks to HUPO talks and events mentioned in this news release are included below), and Dr. Robin Park will discuss IP2 and PaSER at Bruker’s virtual proteomics user’s meeting.
Gary Kruppa博士, Vice President of Proteomics at Bruker Daltonics, commented: “The timsTOF Pro enables 4D-Proteomics with the large-scale measurement of ion mobility yielding collision cross sections (CCS) for every measured peptide. Combined with the timsTOF Pro speed this means that the bottleneck in proteomics has moved from measurement to processing large amounts of data. The speed of IP2 and the PaSER GPU-based search are an ideal match for the timsTOF Pro, and we are thrilled to have Robin Park join us to continue the development of IP2 and PaSER for TIMS/PASEF methods.”
B. Ultra-high Sensitivity and True Single Cell 4D-Proteomics
The groups ofProf. Matthias Mannat the Max Planck Institute in Martinsried, Germany, and at the Medical School of the University of Copenhagen, with collaborators at Evosep and Bruker, have made significant advances in high sensitivity and true single cell proteomics. A modified timsTOF Pro allows robust measurements of proteomes from low sample amounts and even from single cells. Matthias Mann will present his work in “Deep Visual Proteomics for Systems Biology” in the HUPO Connect 2020 program, while his Ph.D. student Andreas Brunner will present at Bruker’s virtual user’s meeting on “Ultra-high sensitivity MS on a timsTOF enables proteomics analysis of single cells – one by one”.
马蒂亚斯·曼恩（Matthias Mann）表示：“从样品处理和测量的角度来看，对真单细胞水平上蛋白质表达的有意义的测量非常具有挑战性。我们很高兴能使Evosep和Bruker成为合作伙伴，以帮助我们实施，证明并最终将我们的想法带到诊所，以便在不久的将来可以向所有研究人员提供真正的单细胞蛋白质组学。”
C.有针对性的定量4D-蛋白质组学和PRM-PASEF
Bruker’s prm-PASEF workflow for quantitative proteomics enables the most highly multiplexed targeted proteomics methods available. The additional dimension of separation provided by TIMS also reduces interferences in MS2 quantitation. Taking advantage of the speed and additional TIMS separation of the underlying PASEF method, prm-PASEF can now target more than a dozen precursors in each 100 msec TIMS separation.Professor Gunnar Dittmar卢森堡大学卢森堡卫生研究院和蛋白质组学教授将出现在布鲁克（Bruker）在Hupo Connect 2020的Bruker虚拟行业研讨会上的“新的PRM-PASEF方法用于临床研究的高度多重靶向定量蛋白质组学”。
D. CAPS-PASEF交联，用于结构4D-蛋白质组学和Phox交联链
Bruker is pleased to announce licensing PhoX cross-linking technology (IMAC-enrichable) as result of the collaboration withAlbert Heckand理查德·谢尔特玛（Richard Scheltema）在乌得勒支大学。TIMSTOF PRO提供了由PHOX提供的交联的优势，并且在“碰撞横截面的辅助前体选择互助质谱法的益处”中描述了新型的CAPS-PASEF方法，该方法在分子和细胞蛋白质组学上发表。Bruker的Phox交联试剂将于2021年初提供。
e。software Developments for Analysis of 4D-Proteomics Data
随着社区利用Bruker独特的开放数据文件格式，Timstof Pro周围的第三方软件生态系统不断增长。新的发展包括Bioinformatics Solutions Inc.的Peaks Studio和Peaks在线软件包的DIA-PASEF的支持。特别是，在线峰值提供了一种增强的基于云的解决方案，用于处理大型样本队列的大型数据集，包括对LFQ定量的进一步改进和SILAC等新工作流程。Maxquant将很快支持DIA-PASEF处理能力。
Biognosys宣布使用Spectromine快速处理TimStof Pro 4D Pasef数据，Spectromine是其用于多重和无标签的定量DDA蛋白质组学的软件解决方案，该解决方案围绕功能强大的PULSAR搜索引擎构建。Dr. Lukas Reiter,Biognosys的首席技术官评论说：“在我们的软件中优化对Timstof Pro的支持是优先考虑的，从而快速处理具有深蛋白质组覆盖率的4D PASEF数据。Spectromine 2现在可以用于等速标记，以及带有Timstof Pro数据的无标签定量（LFQ）工作流程。”
Links to Bruker events at HUPO 2020 can be found here://www.fatactor.com/events-records/2020/hupo-connect-2020.html。
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佩特拉·谢弗（Petra Scheffer）
Bruker Daltonics营销传播
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