Plant Analysis with micro-XRF

Bruker’sM4 TORNADOmicro-XRFspectrometer plays an important role in researching the role of alternative splicing (AS) mechanisms which appear to function in plant responses to environmental stress. Research scientists use theM4 TORNADOto help understand adaptive strategies used by plants to withstand a deficiency or excess of a specific mineral nutrient. This in turn helps determine ways to breed crops with higher mineral nutrient-use efficiency.

Research scientists at Nanjing Agricultural University, Nanjing, China and La Trobe University, Melbourne, Australia collaborated to analyze the role of serine/arginine-rich (SR) proteins which regulate AS mechanisms. They generated mutants of SR protein genes of the rice (Oryza sativa) family to determine if they had a regulatory role in mineral uptake and mobilization in rice shoots. Roots grown in either the presence or the absence of phosphorus (P) were of particular interest.

Distribution of phosphorus in selected leaves was analyzed withmicro-XRFscanning at a high resolution using theM4 TORNADOto confirm that necrotic spots in the leaves only arise under excessive accumulation of phosphorus. This work helped prove that the targets of AS are highly nutrient-specific. It also verified the role of AS in mineral nutrition by characterizing mutants in genes encoding SR proteins that function in AS. Researchers identified proteins as mineral nutrient regulators in rice, showing that three SR protein-encoding genes regulate the phosphorus uptake and remobilization between leaves and shoots of rice, demonstrating that AS has a key role in regulating the mineral nutrient homeostasis in rice.