InVi SPIM Family
Invi Spim产品
Punti salienti
倒视图
The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular structures, and morphological analysis in real-time.
Typical applications of the microscope include dynamic processes in mammalian cell culture applications and even live imaging of intact and living plant models.
Advanced Illumination Module
The Advanced Illumination Module (AIM) is a modular extension that expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample.
添加各种光束图案的选择 - 经典的静态高斯灯表，扫描的高斯梁，贝塞尔束和光学晶格，甚至定制的形状 - 可提供独特的灵活性，以优化您的生物成像实验。
您选择的是3D高分辨率成像实验的最佳结果 - 大型视野，高速或最佳空间分辨率。
Low Phototoxicity and Photobleaching
Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens.
Maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens.
Environmental Control
The InVi SPIM is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
此外，Invi Spim还提供精确稳定的环境控制（即CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.
Sample Holder
将V形长样样品支架放置在腔室内。它被FEP箔覆盖，允许样品与腔室的物理分离，而不会影响成像特性。它的尺寸减小可确保使用小样品中量。
可以使用不同的策略将样品放在持有人中进行成像。2D细胞可以直接在箔上生长，如“弯曲盖玻璃”中。3D球体或小胚胎可以滴入槽中并通过引力固定，从而使多个样品排列以进行多位成像。
The minimization of required specimen medium further leverages its functionality.
Caratteristiche
Inverted, Fast and Flexible
Invi SPIM致力于实时成像，可轻松访问样品室，并控制着生存标本的长期3D荧光成像的环境条件。此外，Invi Spim比旋转磁盘或点扫描共聚焦显微镜要快得多。
它非常适合从细胞培养到早期胚胎（例如果蝇和斑马鱼）的各种样品和应用。
Illumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
该系统具有单方面照明和单镜头检测，从而可以快速获取速度，高分辨率成像。广场成像选项有助于样品定位。
One Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs
The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).
The detection optics are optimized for fast, high-resolution, and very sensitive imaging. The two spectral detection channels equipped with 10 position high-speed filter wheels and a motorized dichroic mirror changer enable simultaneous dual-channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification, and speed.
Compared to other techniques (e.g. confocal laser scanning and spinning disc microscopy), light-sheet microscopy enables the acquisition of a higher number of time points while minimizing photo-damaging effects. A deeper insight into dynamic biological processes and fast movement tracking can be achieved.
Device Design
The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced.
Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.
Applicazioni
Accessori
Accesories
TruLive3D Dishes
InVi SPIM Sample Holder for TruLive3D Dish
Invi Spim标准样品持有人
Software
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
精确的时间控制的连接井斜ces is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- Computer with 256 GB RAM, Intel dual 8 core CPU
- High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
- GeForce RTX 3070 graphics card
- 2 x 10 Gbit/s, 2 x 1 Gbit/s, optional 2x optical port on-board Ethernet port
- 43 inch 4k UHD display