纳米^®

The system performance is maintained through 3 layers of protection:

1. 自动初始化，其中自动采样器和泵在样品注入之前自动运行制备协议，以确保从第一个到最后一个样品的性能。
2. 基于使用模式和集成系统专业知识的预防性维护作业的内置安排，该软件将建议如何优化继续进行系统操作。
3. Precise system diagnostics that automatically detects leaks and blockages and highlights them in the graphical user interface.

根据实验模式，列选择和分析时间，使用Instant Expertiwall™方法编辑器自动生成量身定制的仪器方法：交付：

• LC-MS/MS方法具有智能，自优化的LC分离，以提高生产率和专家口径的结果。
• 最佳的实验条件是根据列类型和样品特征自动计算的，以适合所有蛋白质组学样品
• Chromatographic resolution is preserved for best simultaneous ID and Quant capabilities

梯度可以很容易地修改，但大多数其他参数仍然隐藏，尽管仍然可以在“专家”模式下进行编辑。

The system is preconfigured with a standard layout and a novel valve solution to ensure ease-of-use and system stability. The unique valve design ensures that the user can execute three different types of experiments without hardware changes and software controlled flow paths exist for using a trap column, bypassing the trap column or doing direct infusion for fast analyses of simple samples.

独特的阀设计和新颖的仪器配置启用：

• Software controlled flow paths
• 陷阱柱的动态包含
• 向后冲洗样品以提高峰值

New nano-columns and a unique back-flushable trap cartridge holder complement the instrument and are recognized by the Instant Expertise algorithm.

紧凑而专用的设计with market proven autosampler and large volume, single stroke piston pumps support longer gradients and a wide flow range for efficient Omics knowledge generation.

Modern nano-ESI mass spectrometry (MS) analysis is putting increased pressure on the front-end LC separation to match the MS in terms of robustness and reliability as well as demanding the absolute highest quality and reproducibility of sample separation.

为了确保良好的个人系统性能以及对多个系统和不同实验室的一致性，更深层次的系统集成和新颖的质量控制方法以及智能系统诊断。依次需要对实验的所有阶段进行严格的协议和自动化，将新方法驱动到用户界面设计和数据监视，以及对所有流程路径和配置的液体处理部分的关键重新考虑。

布鲁克纳米乐队^®is designed with years of proteomics know-how and nano-UHPLC expertise.

Based on industry leading components coupled with innovative solutions to common problems, it delivers trouble-free operation from the moment of installation through years of Omics knowledge generation.

当与超高分辨率 /准确的质量QQTOF配对时，Bruker Impact II和市场验证的CaptivesPray电离来源（纳米层）^®当涉及到以下方面的系统是无与伦比的：

• 数据依赖性和独立于数据的自下而上蛋白质组学
• Proteoform profiling for biomarker discovery and validation
• Intact protein characterization
• Glyco- and phosphoproteomics

超高分辨率，准确的质量QQTOF，BRUKER IMPACT II和市场验证的CaptivesPray电离来源是纳米层的理想伴侣^®system.

The complexity of the nanoElute^®内置的智能和应用色谱理解隐藏在直观且易于使用的用户界面后面，但也有效且灵活地操作。

Trouble-free Omics knowledge generation
The nanoElute^®system provides excellent chromatographic performance even for very short gradients in both separation modes. With a trap column installed, the focus is on speed and robustness, however the chromatographic performance is maintained due to new nano-columns and unique back-flushable trap cartridge.

如果没有安装陷阱柱，则可以发射最终的分离功率，而新型阀几何形状的优雅陷阱柱则优雅地通过。

The unique instrument configuration allows the user to analyze samples in both “with trap” and “without trap” mode in the same sample batch without physically changing the hardware setup.

Separation of complex samples with a long column and gradient has been demonstrated both with and without trap column. The retention time and area reproducibility is excellent displaying suitability for all proteomics applications, including the most demanding ones.

方法
Predigested Bovine Serum Albumin (Bruker, Billerica, MA) was used as a representative for a simple proteomics sample (25 fmol/µL).
HeLa cell lysate was prepared according to Beck et al.¹ and acidified with formic acid to a final concentration of 200 ng/µL. For the simple sample, the tryptic peptides were separated on a novel nano-UHPLC system using a 10 min reversed phase gradient (2% - 35% solvent B in 10 min - 0.1% FA in ACN), on standardized 15cm 75µm bore column with C18 resin. The HeLa sample was separated with a reversed phase gradient (from 2% -24% in 90 min, 24-48 in 10 min solvent B - 0.1% FA in ACN), on a standardized 40cm 75µm bore columns with C18 resin. Data was acquired with an impact II QTOF MS (Bruker Daltonik, Bremen, Germany) equipped with a CaptiveSpray ion source..

References
[1] Beck，S.，Michalski，A.，Raether，O，Lubeck，M.，Kaspar，S.，Goedecke，N.，Kulak，N.A.，Cox，J。和Mann，M。“ Impact II，用于深shot弹枪蛋白质组学的非常高分辨率的四极飞行器飞行器（QTOF）。”摩尔细胞蛋白质组学。2015年7月；14（7）：2014-29。

直接输注进行快速分析

The unique valve design also allows ”Direct Infusion” for fast runs of simple samples e.g. BSA at 2 μL/min.

直接输注进行快速分析
The unique valve design also allows ”Direct Infusion” for fast runs of simple samples e.g. BSA at 2 μL/min.