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Multiphoton Microscope

Ultima 2Pplus

用于成像和光学操作的完整的全光照子工作站
Ultima 2Pplus

ハイライト

Ultima 2Pplus

With new advances in field of view, sensitivity, wavelength, and sample accommodation, the Ultima 2Pplus delivers an ideal combination of flexibility, resolution, imaging depth and speed, allowing users to perform simultaneous imaging, stimulation and electrophysiology protocols with greater efficiency and effectivity. The system is designed specifically for intravital imaging, with fully motorized control of the objective X-Y-Z position, as well as two axes of rotation for precise imaging orientation. A second scan path enables simultaneous imaging and photoactivation. The system’s optimized optical train confers exceptional performance to the very edges of the large field.

Class-leading
field of view
Offers a more complete picture with every scan.
High-efficiency
light collection and detection
Images deeper with a greater signal-to-noise ratio.
Simultaneous
imaging and photoactivation
Records cells with exact timing and spatial precision.
Download the Brochure

特長

Features

Largest Field of View and Superior Field Uniformity

The Ultima 2Pplus field of view is over 50% larger to facilitate modern optogenetics experiments, which require simultaneous imaging and photostimulation of distant regions in a sample. A new, custom optical design preserves spatial and temporal resolution throughout the entire field, which is essential for researchers to be able to continue their work without changing experimental designs.

Field of view on Ultima 2Pplus is FN 28 mm in comparison to industry standard of FN 18 mm.

High-Sensitivity Deep Imaging

1.3mm deep, 3D-view of pyramidal cells in layer 5. Data collected in one-month-old transgenic Thy1-YFP-H line mouse through a glass-covered cranial window. Data courtesy of Guang Yang, School of Medicine, New York University, New York.

The Ultima 2Pplus light collection path ensures detection of almost all photons scattered from dim, thick and opaque biological samples. The superior collection efficiency is provided with 2-inch or larger optics
throughout the emission path up to the sensitive close-proximity detectors. As two-photon microscopy has become well adopted, emerging three-photon microscopy offers the potential to image even deeper into
samples. Since further infrared wavelengths are scattered even less, three-photon excitation has the promise to be able to image several millimeters into the brain. Ultima 2Pplus already uniquely incorporates this capability to take your research to new levels of potential.

Perfected Photostimulation and Imaging

The Ultima 2Pplus empowers sophisticated optogenetics experiments with Bruker's Neuralight 3DTMSpatial Light Modulator option. Large populations of targets can be stimulated simultaneously in multiple focal planes with computer-generated holograms. During 3D holographic experiments, targets at different depths are imaged with a custom-designed, optically-corrected ETL focusing module that allows for independent Z-positioning of the imaging plane relative to photostimulation.

Optical stimulation of circled cells with Neuralight 3D. 3D hologram of points was created which was then spirally scanned over neuronal cell bodies. Data Courtesy of Adam Packer, Lloyd Russell, Henry Dalgleish, Michael Häusser’s lab, UCL, London.

アプリケーション

Applications

Ultima 2pplus数据库

1.345 mm x 1.345 mm FOV, resonant scanned image of GCaMP expressing cells in mouse brain.
Two-photon imaging of cortical surface with Ultima 2Pplus through a cranial glass window (grey). Acquired with Nikon 16x 0.8 NA objective at 920 nm. Data courtesy of Dustin Herrmann, Mehmet Fisek, Michael Häusser’s lab, UCL, UK.
Co-expression of GCaMP6s (green) and soma-restricted C1V1-mRuby2 (magenta) in layer 5A neurons of mouse visual cortex at a depth of ~400 µm below the pia (right). Both acquired with Nikon 16x 0.8 NA objective at 920 nm. Data courtesy of Dustin Herrmann, Mehmet Fisek, Michael Häusser’s lab, UCL, UK.
Spontaneous fluorescence fluctuations in neurons expressing GCaMP6 in transgenic mouse. Data courtesy of Robert Lees, James Rowland, Adam Packer Lab, University of Oxford, UK.
Large FOV montage (44 on scope with FN28 vs 108 tiles on scope with FN18) of hippocampal slice and details of selected tile. In-house data.
3D-view of volumetric stack recorded using the ETL focusing module. Layer 5B neurons in mouse visual cortex in vivo, labelled with tdTomato. Scale bar 100 µm. Data courtesy of Lisa Bauer, Dustin Herrmann, Mehmet Fisek, Michael Häusser’s lab, UCL, UK.
3D structural imaging of hippocampal slice. In-house data.
Co-expression of GCaMP6s and soma-restricted C1V1-mRuby2 in layer 5A neurons of mouse visual cortex. Acquired in vivo, ~400 µm below pia at wavelengths 920 nm and 765 nm. Data courtesy of Dustin Herrmann, Mehmet Fisek, Michael Hausser's lab, UCL, UK
Holographic photostimulation of neurons expressing GCaMP6 and ChRmine in OFC through 4.2 mm grin lens. Data courtesy of Vijay Namboodiri, Charles Zhou, Garret Stuber Lab, University of Washington, WA
两光子激活导致焦点收缩。SLM聚焦在红色箭头指示的位置上的6个斑点。总刺激功率约为200 mW，在容器两侧的6个斑点上分布在6个斑点上。将每个位置在直径12μm的螺旋中扫描100 ms。白色箭头用于比较。船只被德克萨斯州红葡萄兰标记。速度4x实时。数据由乔治亚州奥古斯塔奥古斯塔大学的Phil O’Herron提供。
Comparison of FOV between 1x zoom (left) and 0.8x zoom (right) in galvo-galvo mode
Constriction of a penetrating arteriole with the 594 nm LED. 5 pulses, 100 ms duration with 100 ms in between were presented. Speed 4x real time. Data courtesy of Phil O’Herron, University of Augusta, Augusta, GA.
藉由此激活PDG与领导的594海里FRβ/ReaChR mouse. Image is looking down at pial surface vessels in the neocortex labelled with FITC dextran. A single 100 ms pulse from the 549 nm LED was used to stimulate the vessels. The dark flash indicates the time of the optical stimulation. The image darkens because the detectors are briefly blocked by mechanical shutters to protect them from the intense stimulation light. Speed 4x real time. Data courtesy of Phil O’Herron, University of Augusta, Augusta, GA.
小鼠皮肤中表达EGFP的树突状表皮T细胞（绿色）。静脉注射的Rhodamin 6G划定皮肤表皮边界（红色）。毛轴（紫色）植根于毛囊中，其皮脂腺（蓝色自动荧光）揭示出来。数据由MGH哈佛大学Thorsten Mempel博士提供。
CD11C-MCHERRY转基因报告基因小鼠的女性生殖道中表达麦克网的树突状细胞（红色）。该动物被静脉注射Hoechst 33342，以染色所有成核细胞（蓝色）。上皮细胞用自动荧光信号（绿色/绿松石色调）可视化。数据由MGH哈佛大学Thorsten Mempel博士提供。
小鼠皮肤中表达EGFP的树突状表皮T细胞（绿色）。静脉注射的Rhodamin 6G划定皮肤表皮边界（红色）。毛轴（紫色）植根于毛囊中，其皮脂腺（蓝色自动荧光）揭示出来。数据由MGH哈佛大学Thorsten Mempel博士提供。
GFP-expressing T cells (green) accumulating around a pancreatic islet in a mouse developing autoimmune pancreatitis ultimately leading to diabetes. Data courtesy of Dr. Thorsten Mempel, MGH Harvard.
表达GFP的细胞毒性T淋巴细胞（绿色）和表达TDTOMATO的调节性T细胞（红色），浸润植入背面皮肤折叠室的小鼠结肠癌。肿瘤细胞核（蓝色）通过表达Cerulean-Histone H2B融合蛋白标记。血管（白色）通过静脉注射QTRACKER 655量子点突出显示。数据由MGH哈佛大学Thorsten Mempel博士提供。

仕様

Specifications

SCAN HEAD
Scanning Method Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities
Field of View ~1.375 mm x 1.375 mm with 16x objective (≤28 mm FN)
扫描速度

Raster scan: 1.65 fps at 512 x 512, >12 fps at 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64
Scan Customization User-definable straight, freehand and circular (infinite) linescan with included software; User-definable pixels/line and lines/scan from 1 to 2048; ≤120x scan zoom; 360° of scan rotation; Point scan
Uncaging Option 第二组匹配的3毫米或6毫米剑桥电量计在同一扫描头中提供高精度可见或多光子激光样品光掌声
High-Speed Imaging Option 8 kHz resonant galvanometer; ~30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest
探测器
Reflected Non-Descanned 1 to 4 hand-picked Hamamatsu Multi-Alkali PMTs; Upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Transmitted Non-Descanned 1 or 2 hand-picked Hamamatsu Multi-Alkali PMTs; Upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Dodt Single Hamamatsu PMT for DIC-like image collection
Transmitted Single Hamamatsu PMT for transmitted light image collection
Camera 扫描头部内置的标准C安装相机端口
OPTICAL INPUTS
Multiphoton Laser Optimized for multiphoton laser input from 690 to 1700 nm
Epifluorescence High-Powered LED in epifluorescence turret
Visible Laser Fibered laser inputs for visible laser introduction
Ultima Laser Rating Class 1 (Contains Class 3b and Class 4 lasers) with light box
Helios Laser Rating Class 3b
LED 带有LED模块的全场光活化
MOTOR CONTROL
Bruker X, Y Stage Variable height with ~15 cm X and ~7.5 cm Y movement and ~0.3 μm step size
Bruker X, Y Microscope Base Fine and coarse-movement platform for scope with ~35 mm travel and 0.1 μm step size
Bruker Z-Focus 范围为30毫米，步长约0.2μm
Bruker Z-Piezo Travel range ≤1000 μm with 0.05 μm step size
Bruker Orbital Nosepiece Motorized control of objective angle and rotation
SOFTWARE
Prairie View Imaging Turnkey intuitive and customizable operation for imaging
Z-Series 轻松地创建depth stacks with user-customizable slice number, step size and laser power
T-Series 轻松地创建complex series involving Z-Series and triggered images
Stage Montage Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages
Peripherals Integration Wavelength and power control available for multiphoton and visible laser launches
Photoactivation User-defined points and regions with synchronized laser modulation
Regions of Interest User-defined regions for faster scanning capabilities
Brightness Over Time 随着时间的推移，用户定义区域的强度映射和绘图
Voltage Inputs/Outputs Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with external devices

ウェビナー

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お客様の声

Testimonials

Hear What Our Customers Have to Say

We have the 2Pplus - the scope is well-designed and the software easy to use. Their scopes are very flexible in design if you wanted to add additional optics, components, or software functionality. Bruker shines with respect to customer service… they have several great service experts who usually respond within a day or two with insightful solutions. Further, the support is highly personalized; not before long after purchasing the microscope, we were paired with specialists who intimately familiarized themselves with our applications.

查尔斯周（Charles Zhou）博士，上瘾，痛苦和情感的神经生物学中心

我们欣赏Ultima硬件和软件的模块化性质。现在可用的系统范围从基本成像系统到双激光刺激多模式成像工作站。我们继续更新14个机器中的许多，包括我们十年的系统。团队观众的连接和支持对于新手所有者和重大软件更改是一项巨大的资产。

西北大学大卫·沃科辛（David Wokosin）博士

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