Multiphoton Microscope

Ultima In Vitro

A timeless workhorse for simultaneous multiphoton imaging and photoactivation (a.k.a Prairie Scope)
Ultima In Vitro

하이라이트

Ultima In Vitro

Ultima体外多光子显微镜系统是领先的科学家在与神经信号传导和神经网络映射有关的脑部滑板研究中进行突破实验的平台。Ultima在体外提供同时的成像和电生理学,以及当今神经科学家所需的完整刺激和记录环境的未整体和光刺激。

Secondary
scan path
Enables simultaneous imaging and photostimulation.
亚微米
positioning control
Allows point photoactivation.
Precise
触发
Integrates with electrophysiology systems.

특징

Features

Flexible Architecture to Meet Varying Requirements

Ultima In Vitro is the ultimate “slice rig," having provided the platform on which leading scientists have conducted breakthrough experiments in brain slices related to neural signaling and neural networks for more than ten years. The system provides the flexibility for the most demanding protocols, and is available on upright or inverted optical microscopes. Features include:

  • 辅助扫描路径,可以同时进行成像和光刺激
  • Visible, UV, and IR inputs for photostimulation
  • 亚微米positioning control for point photoactivation
  • Sub-millisecond spirals for somal actiavtion
  • 同步和触发以同时进行成像,光刺激和电生理学
  • Integrated electrophysiology control and recording with up to 16 analog outputs/inputs
Hippocampal pyramidal neuron and voltage recordings after uncaging.

High-Resolution Multifield Imaging

5x5 montage at 40x (covering area of 1175 um x 1175 um) of mouse kidney section with Alexa Fluor® 488 WGA, Alexa Fluor® 568 Phalloidin.

The Atlas Imaging module in Prairie View software simplifies setup of 2D and 3D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3D montages can be variable, allowing more efficient collection of data.

Pharmaceutical Studies with Tissue Explants

Tissue explants provide a higher throughput model for studying tissue response to pharmaceutical agents. The FLIM option allows measurement of metabolic status through measurement of fluorescent lifetimes.

Quantitative optical imaging of primary tumor organoid metabolism predicts drug response in breast cancer. AJ Walsh, RS Cook, ME Sanders, L Aurisicchio, G Ciliberto, CL Arteaga, and MC Skala. Cancer Research (2014). Sep 15;74(18):5184-5194.

Neuronal Signaling in Brain Slices

EPSP recorded in the dendritic trunk in response to a single, high-power ungaging event at s1 (black) or s2 (grey) in brain slice; Mark T. Harnett et al., Nature 491, 599-602.

Ultima In Vitro is the system of choice for studying neuronal signaling at the both the dendritic and neural network levels. When equipped with optional photostimulation galvanometers, Ultima In Vitro provides a complete optical workstation, allowing simultaneous imaging, photostimulation and electrophysiology recording.

细胞制剂的体积成像

Ultima In Vitro provides high resolution and efficient volumetric imaging of cell cultures and cells in artificial matrices.

EPSP recorded in the dendritic trunk in response to a single, high-power ungaging event at s1 (black) or s2 (grey) in brain slice; Mark T. Harnett et al., Nature 491, 599-602.

사양

Specifications

SCAN HEAD
Scanning Method Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities
Field of View ~600 μm x ~600 μm with 20x objective
扫描速度 Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64
Scan Customization 用户自行设定直,徒手画的and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan
Uncaging Option Second set of matched 3 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample ablation
High-Speed Imaging Option 8 kHz谐振电流计;30 fps在512 x 512,> 1300 fps在512 x 8的兴趣区域
共聚焦选项 Point scan: Automated pinhole confocal attachment with up to three PMTs
多点scan: Camera-based for high-speed imaging
DETECTORS
Reflected Non-Descanned 1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
传输非散布 1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
多特 单个Hamamatsu PMT,用于类似DIC的图像收集
Transmitted Single Hamamatsu PMT for transmitted light image collection
Confocal 点扫描:1-3个手工挑选的Hamamatsu Multi-Akali PMT;可升级到高敏性hamamatsu gaasp PMTS
多点:EMCCD
Camera 标准C2相机端口内置在标准OLM图像收集的扫描头中
光学输入
Multiphoton Laser Ultima light path is optimized for multiphoton laser input from 690 - 1300 nm
Epifluorescence Standard epifluorescence head included for fluorescence imaging
Visible Laser 可见激光介绍的纤维激光输入
Ultima激光额定值 Class 1 (Contains Class 3b & Class 4 lasers)
Helios激光额定值 Class 3b
引领 Full-field photoactivation with LED module
PLATFORM AND AUTOMATION
显微镜 Nikon Ti-E inverted and Nikon Ni-E upright; Olympus BX51WI and BX61WI; Zeiss Axio Observer and Axio Examiner
Specimen Stage Bruker Slim Stage for upright; ASI stage with piezo insert for inverted
Z-Focus TI-E,BX 61和Zeiss的内部聚焦电机;BX51WI和NI-E的Bruker Z-Gocus Motor;BRUKER Z-PIEZO在BX51WI,BX61WI和NI-E上可用
SOFTWARE
Prairie View Imaging 与扫描头完全集成​​的软件,可轻松成像;可自定义的扫描设置以优化样品激发;激光功率和PMT高压的集成控制
Z系列 Easy creation of depth stacks with user customizable slice number, step size and laser power
T-Series Easy creation of complex series involving Z-Series and triggered images
Stage Montage Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages
外围物集成 Wavelength and power control available for multiphoton and visible laser launches
光激活 User-defined points and regions for complex laser blanking
Regions of Interest User-defined regions for faster scanning capabilities
Brightness Over Time Capable of collecting BOT information for user-defined regions over time and/or depth
Voltage Inputs/Outputs Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with internal devices

부속품

配件

Ultima In Vitro Accessories and Options

Resonant Scanner
  • Provides high-speed acquisition of 30 fps at 512 x 512
  • Relayed beam path utilizing X and Y galvos enables ROIs in X and Y, which enables panning of ROIs and higher S/N ratio
  • Can be used with photostimulation path for simultaneous high-speed imaging and stimulation
  • Read More
Photostimulation Path
  • Provides capability for simultaneous photostimulation or uncaging with imaging
  • Available for fast pulsed IR, UV or visible laser inputs
  • Precise synchronization with image and external triggers
Fluorescence Lifetime Imaging (FLIM)
  • Provides FLIM acquisition integrated with all raster scan galvo modes and ROIs
  • Enables the inclusion of FLIM experiments in Z and T-Series
Piezo Focus
  • Provides high-speed z-positioning with .05 μm accuracy
  • Focus over a 150 μm range
  • Unique calibration enables continuous movement for no dead-time during z-series acquisitions
双波长模块
  • Provides hardware and software components for imaging with two different multiphoton wavelengths simultaneously (with two lasers)
  • Software options allow for interlaced or simultaneous excitation scanning
  • 激发多个具有不同激发波长的探针,而无需停机激光调整
Full-Field Optical Input/Output Module
  • Provides full-field access to the imaging axis for light stimulus introduction or widefield imaging
  • Variable zoom capability

웨비나

추천 글

推荐

Hear What Our Customers Have to Say

We appreciate the modular nature of the Ultima hardware and software. The systems now available range from basic imaging systems to the dual-laser photo-stimulation multi-mode imaging workstation. We have continued updating many of our 14 machines, including our ten year old systems. The Team Viewer connection and support is a huge asset for novice owners and for significant software changes.

David Wokosin, Ph.D., Northwestern University

전문가에게 문의하십시오.

Contact Us

* Please fill out the mandatory fields.

请输入您的名字
请输入您的姓氏
请输入您的电子邮件地址
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
请接受条款和条件

이 사이트는 Google과 reCAPTCHA의 보호를 받습니다.개인 정보 보호 정책서비스서비스지원하다.