Ultima体外多光子显微镜系统是领先的科学家在与神经信号传导和神经网络映射有关的脑部滑板研究中进行突破实验的平台。Ultima在体外提供同时的成像和电生理学,以及当今神经科学家所需的完整刺激和记录环境的未整体和光刺激。
Ultima In Vitro is the ultimate “slice rig," having provided the platform on which leading scientists have conducted breakthrough experiments in brain slices related to neural signaling and neural networks for more than ten years. The system provides the flexibility for the most demanding protocols, and is available on upright or inverted optical microscopes. Features include:
The Atlas Imaging module in Prairie View software simplifies setup of 2D and 3D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3D montages can be variable, allowing more efficient collection of data.
Tissue explants provide a higher throughput model for studying tissue response to pharmaceutical agents. The FLIM option allows measurement of metabolic status through measurement of fluorescent lifetimes.
Ultima In Vitro is the system of choice for studying neuronal signaling at the both the dendritic and neural network levels. When equipped with optional photostimulation galvanometers, Ultima In Vitro provides a complete optical workstation, allowing simultaneous imaging, photostimulation and electrophysiology recording.
Ultima In Vitro provides high resolution and efficient volumetric imaging of cell cultures and cells in artificial matrices.
SCAN HEAD | |
Scanning Method | Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities |
Field of View | ~600 μm x ~600 μm with 20x objective |
扫描速度 | Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64 Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64 |
Scan Customization | 用户自行设定直,徒手画的and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan |
Uncaging Option | Second set of matched 3 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample ablation |
High-Speed Imaging Option | 8 kHz谐振电流计;30 fps在512 x 512,> 1300 fps在512 x 8的兴趣区域 |
共聚焦选项 | Point scan: Automated pinhole confocal attachment with up to three PMTs 多点scan: Camera-based for high-speed imaging |
DETECTORS | |
Reflected Non-Descanned | 1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
传输非散布 | 1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
多特 | 单个Hamamatsu PMT,用于类似DIC的图像收集 |
Transmitted | Single Hamamatsu PMT for transmitted light image collection |
Confocal | 点扫描:1-3个手工挑选的Hamamatsu Multi-Akali PMT;可升级到高敏性hamamatsu gaasp PMTS 多点:EMCCD |
Camera | 标准C2相机端口内置在标准OLM图像收集的扫描头中 |
光学输入 | |
Multiphoton Laser | Ultima light path is optimized for multiphoton laser input from 690 - 1300 nm |
Epifluorescence | Standard epifluorescence head included for fluorescence imaging |
Visible Laser | 可见激光介绍的纤维激光输入 |
Ultima激光额定值 | Class 1 (Contains Class 3b & Class 4 lasers) |
Helios激光额定值 | Class 3b |
引领 | Full-field photoactivation with LED module |
PLATFORM AND AUTOMATION | |
显微镜 | Nikon Ti-E inverted and Nikon Ni-E upright; Olympus BX51WI and BX61WI; Zeiss Axio Observer and Axio Examiner |
Specimen Stage | Bruker Slim Stage for upright; ASI stage with piezo insert for inverted |
Z-Focus | TI-E,BX 61和Zeiss的内部聚焦电机;BX51WI和NI-E的Bruker Z-Gocus Motor;BRUKER Z-PIEZO在BX51WI,BX61WI和NI-E上可用 |
SOFTWARE | |
Prairie View Imaging | 与扫描头完全集成的软件,可轻松成像;可自定义的扫描设置以优化样品激发;激光功率和PMT高压的集成控制 |
Z系列 | Easy creation of depth stacks with user customizable slice number, step size and laser power |
T-Series | Easy creation of complex series involving Z-Series and triggered images |
Stage Montage | Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages |
外围物集成 | Wavelength and power control available for multiphoton and visible laser launches |
光激活 | User-defined points and regions for complex laser blanking |
Regions of Interest | User-defined regions for faster scanning capabilities |
Brightness Over Time | Capable of collecting BOT information for user-defined regions over time and/or depth |
Voltage Inputs/Outputs | Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with internal devices |
We appreciate the modular nature of the Ultima hardware and software. The systems now available range from basic imaging systems to the dual-laser photo-stimulation multi-mode imaging workstation. We have continued updating many of our 14 machines, including our ten year old systems. The Team Viewer connection and support is a huge asset for novice owners and for significant software changes.