Combined with Bruker's software-controlled, automated, fully-integratedMicrofluidics Unit, 这Vutara VXLsuper-resolution microscope is唯一的商业系统that offers out-of-the-box multiplexed DNA-PAINT capabilities. Performed on these tools, DNA-PAINT imaging allows for sub-10 nm localization precision in single-molecule localization microscopywith unlimited multiplexing potential。
Moreover, the Vutara microscope with microfluidics system provides the added benefits of:
DNA-PAINT is a technique to enable single-molecule localization through the binding of short (<10 nucleotides) oligonucleotides labeled with a fluorophore to a complementary oligonucleotide bound to a target molecule, typically an antibody. It allows for extensive imaging modalities, from whole-cell extensive Z-stacks to high-resolution multi-target images.
DNA-PAINT通过短暂的“成像寡核苷酸”的瞬时结合,该结合含有荧光团到互补的寡核苷酸(称为“停靠链”)的目标,例如感兴趣的目标,例如抗体,纳米体,Aptamer,Aptamer或suicide酶体。
Super-resolution imaging with DNA-PAINT involves several steps, including:
由于样品被沐浴在大量不断交换成像链中,因此目标本质上是不可漂白的,因此可以将大量框架和扩展的Z堆栈进行分解。
DNA-PAINT allows sub-10 nm localization precision, making it one of the most precise microscope techniques available.
在这里,将带有水浸入水1.2 Na物镜的Vutara显微镜用于DNA-Paint实验。该图像显示了整个BS-C-C-1细胞的小管蛋白网络,该网络用与DNA-Paint二抗结合的微管蛋白抗体标记。插图显示了微管蛋白网络的放大部分。微管的管腔清晰可见。
通过DNA-Paint使多重多色超分辨率成像成为可能。
DNA-PAINT has the potential for large-scale multicolor Z-stacks due to the fact the sample is bathed in a practically limitless supply of fluorophore. This enables large-scale z-stack imaging composed of millions of localizations.
Here, a two-color DNA-PAINT experiment was performed on the Vutara single-molecule localization microscope. Tubulin is labeled in cyan and clathrin in magenta. Furthermore, due to the unbleachable nature of DNA-PAINT large Z-stacks are possible.
DNA-PAINT has enormous potential for multiplexed imaging.
在这里,使用VUTARA VXL和集成的流体单元进行了多目标DNA-Paint实验。在不同的探针上使用正交对接链,可能有可能无限的目标数量。
还图所示是在Vutara单分子定位显微镜和集成的流体单元上进行的四个目标DNA漆实验。F-Actin-Magenta,Tom20-Cyan,微管蛋白 - 黄色和网状蛋白绿色。
BS-C-1用抗微管蛋白,肌动蛋白,抗TOM20和抗克拉蛋白标记。购买正交2ºDNA涂料是从中购买的巨大的 - photonics.com。