amaZon speed ETD

The amaZon speed ETD incorporates key inventions for unprecedented proteomics capabilities. The high dynamic range and complexity of proteomics samples due to post translational modifications require maximum speed and sensitivity to capture as many peptides as possible.

The amaZon speed ion ETD trap sets new standards in protein identification due to its enhanced MS/MS speed, new SWIFT isolation and SMART fragmentation.

This results in routine identification of more than 2500 unambiguously identified proteins in a single LC-MS/MS run from 5 µg of a HeLa lysate. The dual ion funnel transfer line is delivering absolute benchmark sensitivity.

amaZon speed ETD offers:

• Bottom-up proteomics identifying more low abundant proteins due to its greatly increased MS/MS duty cycle
• Top-down sequencing (TDS) for the highest protein sequence coverage
• Market leading mass accuracy for confidence in results
• 领先的双离子漏斗技术，用于无与伦比的灵敏度
• Outstanding ETD/PTR applications for full post-translational modification (PTM) analysis
• SMART isolation and fragmentation
• Unrivaled scan speed of 52,000 u/sec at a peak width < 0.5 u for resolution of doubly charged ions
  

High sequence coverage by CaptiveSpray
The amaZon speed is equipped with the high-transmission, robust CaptiveSpray source for high performance and reproducibility in bottom-up studies.

Ultimate protein identification rates
The amaZon speed offers a survey view of a Hela cell digest (5 µg) separated by a nano UHPLC and amaZon speed ETD equipped with a CaptiveSpray ion source.

市场领先动态范围
The amaZon speed ETD identifies proteins over at least five concentration levels in a single run.

Many proteins execute their molecular function by reversible posttranslational modifications (PTMs), like phosphorylation for example. The identification and unambiguous localization of the modification site is of major importance to understand the biological context. ETD preserves the modification of the peptide backbone and therefore allows clear assignment of the modification site. The amaZon speed offers unrivaled performance through its proven sensitive, robust and reliable ETD technology.

Understanding relationships between structure, location, and function of glycoproteins requires detailed information about peptide sequence, glycan composition, and glycosylation sites. A wide variety of analytical approaches are employed to analyze intact glycoproteins, glycopeptides, and released glycans, with mass spectrometry playing a major role.

已经证明了使用碰撞诱导的解离（CID）和电子传递分离（ETD）在简单糖蛋白消化中分析N-糖基化肽的亚马逊速度ETD系统的性能。

在数据采集过程中，CID和Oxonium-ION触发的ETD的有效组合 - “片段触发的ETD” - 即使在复杂的混合物中，也可以在单个运行中进行广泛的蛋白质序列鉴定和详细的糖肽分析。

在Amazon Speed ETD平台上触发ETD的Activespray纳米生物体电离源和片段大大增强了蛋白质混合物中的糖肽分析。