Surface plasmon resonance (SPR)

Sierra Spr-24 Pro

行业领先的SPR系统提供从测定开发到初始筛选的最高性能

高通量

八个频道/三个位置的数组启用了24小时内的8800互动

Sierra-SPR-24-Pro

najważniejszeInformacje

Surface plasmon resonance (SPR)

Tailored for high-content applications

灵活性
选择1到8针的任何组合,以获得最佳性能,灵活性和应用范围。
Robust
旨在执行所有SPR应用,并能够处理细胞裂解物等粗样品。
24个传感器点
Simultaneous analysis of up to 8 samples injected over 24 sensor spots.
生产率
The 8x3 channel/spot array allows multiple controls or targets on a single sensor chip for maximum read out per single sample injection.

Cechy Charakterystyczne

Sierra Spr-24 Pro

分析生物传感器,用于实时的,无标签的分子相互作用

Continuous Flow SPR Analysis
The SPR-24 Pro system is a continuous flow biosensor, delivering sample and reagents to the detection sensors as continuously flowing streams. When sample or reagent injection stops, a “continuous flow buffer” is passed over the sensors.

SPR分析中连续流缓冲区的配方可能会对要测量的分子相互作用产生重大影响。在许多应用中,需要在各种缓冲环境中测量目标相互作用。

Sierra Spr-24 Pro

框架注入

The Frame Inject feature in the Sierra SPR-24 Pro system allows the investigation of condition dependent binding / mode of action studies. The feature requires just a fraction of each buffer condition that replaces the system buffer while usage. The pre-association, as well as the dissociation occurs under the conditions, that should be investigated. After dissociation end, frame solution buffer is replaced and system buffer is flowing through the microfluidic system again. Thus experimental time and reagent costs are kept low. The same buffer can be directed to all flow cells, or up to eight different buffers can be assayed simultaneously.

框架注入allows the investigation of up to eight buffer conditions in parallel.
Sierra Spr-24 Pro

通过单个针头控制最大化性能

Individual Needle Control
Sierra Spr-24 Pro系统中的单个针控(INC)功能允许独立操作八个样品拾音针头。研究人员可以灵活地选择1-8针的任何组合来设计其最佳测定法。

单个针头控制可最大程度地提高Sierra Spr-24 Pro的应用程序,灵活性和范围,同时还可以节省时间,材料和资源,当时吞吐量不是主要要求。必威手机客户端

The eight-needle probe head can simultaneously process 8 sample or reagent solutions. Using the INC feature, users can choose to use any number and/or combination of needles at any time during analysis.

Korzyści

Sierra Spr-24 Pro

Throughput on demand

The Sierra SPR-24 Pro delivers faster throughput across a wide range of applications, from kinetic assays with a different target on each sensor to detailed analysis employing multiple in-line controls.

The system uses Bruker’s Hydrodynamic Isolation (HI) continuous flow microfluidic technology to address samples as flowing streams onto the 24 spot SPR+传感器阵列。当与可选的板处理机器人集成时,Sierra Spr-24 Pro仪器每天可以测定超过4400个样品,产生超过8800个控制减去结合响应。

Sierra Spr-24 Procan be easily integrated into an automated scheduling software via API.

Sierra Spr-24 Pro

Throughput with performance

灵活的Sierra Spr-24 Pro系统是高通量应用的理想选择。它提供24个检测点,由8个注射针来解决。单个针控(INC)使研究人员可以选择选择1到8针的任何组合,以优化应用程序的性能,灵活性和范围。

24传感器检测阵列在8个流动电池中布置,每个流动池包含3个传感器。可以同时分析多达8个样品,每个分析周期最多生成24个结合测量。所有24个传感器也可以完全独立于其他传感器,从而提供最大的测定设计灵活性。

多功能系统还可以节省实验室中的时间和材料,尤其是在吞吐量不是主要要求的情况下。必威手机客户端

使用框架注入功能,可以在仪器中同时使用多达八个缓冲区。在单个测定中评估不同pH值,离子强度,清洁剂或溶剂浓度以及副因素或抑制剂的效果的能力会导致更简单,优化的测定最终提高吞吐量和生产率。每个通道设计的四传感器设计为控制分析提供了最大的灵活性,同时为最复杂的分析保持吞吐量。

Sierra Spr-24 Pro

提高生产率

Sierra Spr-24 Pro的设计最大化了控制分析的灵活性,为最复杂的分析提供了高吞吐量。可以在两个控件和活动传感器上同时分析样品。

对非特异性结合与基质蛋白(如HSA或BSA)的早期分析可能至关重要。额外的在线控制不仅将所需的测定循环的数量减半,还可以通过消除多个样品制剂之间的潜在变化来提高数据质量。

From initial assay development to pre-defined method templates, the Sierra SPR Control Software provides the perfect solution for every use case. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well-defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.

MateriałyEksploatacyjne

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Sierra Spr-24 Pro

表面等离子体共振的应用


使用框架注射对条件依赖性结合的研究

The traditional investigation of condition-dependent binding demands high reagent costs as well as lengthy run time. Frame inject delivers cost and time savings at low sample consumption.


如何使用胺耦合影响配体密度

找到理想的固定水平以获得最佳的动力学测量至关重要。在这里,我们检查了不同示例目标上的影响参数。


Screening and characterization of small molecule binding to protein targets

通过SPR检测的实时,无标签(RT-LF)分析是小分子药物和候选药物的生物物理表征的强大工具。


筛选和表征生物治疗学

通过SPR检测的实时无标签(RT-LF)分析是蛋白质疗法生物物理表征的强大工具。