timsTOF fleX
Patialomx®驱动
ESI/MALDI with Zero Compromises
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MALDI Guided SpatialOMx®on the timsTOF fleX
Особенности
From 4D-OMICS to molecular imaging without stopping, changing, modifying or compromises
Dual source design to matchPasef®empowered LC-MS/MS identification with label-free spatial localization to decode the molecular make up of your sample
Built on the standard for shotgun proteomics, the timsTOF fleX combines best in class 4D X-Omics with Bruker’s cutting edge MALDI Imaging technology, includingsmartbeam3D laser optics for fast measurement all in one platform. A dual source instrument ideal for SpatialOMx®, timsTOF fleX conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. No single instrument has before provided access to both essential capabilities for the most advanced OMICS researchers.
During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds. No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class OMICS identification and quantification workflows to creating high definition molecular maps of tissue sections to see what matters most.
真正的像素成像高速和高空间分辨率
True pixel imaging is key to get molecular insights into histological regions or even into single cells. The smartbeam 3D laser technology implemented in the timsTOF fleX enables fast acquisitions and retains true pixel shapes.
Irregularities in thickness of tissue sections do not influence mass accuracy in the QTOF configuration of the timsTOF fleX since the mass analyzer is decoupled from the ion source and leads to high-definition MALDI images.
Zoom mode to see what really matters
Besides the ability to generate high definition “True Pixel” MALDI images, the smartbeam 3D laser provides rock solid laser guided targeting down to 10 µm with Bruker’s new zoom mode technology. Users now have the flexibility to adjust their laser spot size and spacing to accommodate a wider range of granularity depending on biological features of interest.
通过在MALDI图像中添加额外的维度来深入研究
自谱产生的离子和ESI旅行same path to the detector from the source, MALDI workflows can take advantage of the most advanced features found in the timsTOF Pro, including trapped ion mobility separation (TIMS) based on the collisional cross section (CCS) of detected molecules. Tuning and calibration can be performed in ESI mode and used for the MALDI experiment for additional ease of optimization.
TIMS允许分离分子取决于离子的形状。离子与气流一起进入双重TIMS设备，并通过电场在第一部分中积累。实际分离发生在Tims墨盒的第二部分。通过降低电势以时间和空间方式洗脱离子。可变坡道速度和移动范围的适应可为不同类别的分子进行优化，从而为用户提供了高灵活性。
Преимущества
Bring a spatial dimension to X-Omics analyses
将MALDI成像的重新特定与深入的X-Momics分析相结合从未如此容易。MALDI成像适用于检查广泛的分析物，包括代谢产物，脂质或聚糖以及与显微镜工作流无缝兼容，MALDI成像可以识别出时空特异性的化合物分布®分析。具有双源设计的尖端仪器与证明的高纯度消耗品搭配使用，可用于强大的分析和用户友好的软件，可节省时间，使您的研究生活更轻松。使用布鲁克的Intellislides用于自动化的MALDI成像和PastialOmx®workflows on the timsTOF fleX.
Take advantage by separating isobaric or isomeric ions
The analyses of complex samples such as tissue sections benefit from trapped ion mobility spectrometry (TIMS). Separate isobaric or isomeric metabolites, lipids, peptides or glycans to get the true spatial localization of your analytes. The timsTOF flex offers the only chance to differentiate isomeric distributions where high mass resolution fails.
使用碰撞横截面（CCS）作为TIM的结果，以额外的质量标准验证分析物的身份。CCS感知软件智能地将空间MALDI-TIMS成像数据与X-Momics结果匹配，并为ID列表提供了重要的形态环境。
Going from the chromatogram to the pixel has never been easier …
Timstof Flex是一种用于探测样品分子含量的多功能仪器。Built on Bruker’s pioneering timsTOF Pro platform, the timsTOF fleX is a fully functional high speed, high sensitivity ESI instrument for all your X-Omics analyses, combined with a high spatial resolution MALDI source and stage specifically designed for resolving molecular distributions and bringing a spatial dimension to OMICS analyses. Transform proteomics analysis into spatial proteomics,lipidomicsinto spatial lipidomics, andmetabolomics into spatial metabolomics and get tissue context to your data. Interact with other disciplines and get more out of your analytical data to be successful.
“Ease of Use” workflows for Imaging beginners
Our ready-to-run consumables and software products enable you to acquire and interpret molecular tissue images like a seasoned professional. Automated imaging runs built on our advancedIntellislides™节省时间和精力，并确保确切的结果。可重现的结果可以保证我们的纯净fleXmatrix™provided in pre-measured vials ready for coating. Get access to these consumables and more in Bruker’s webshop.
Converting raw data into statistically treated information and enlightening images is carried out bySCILS™实验室, the “gold standard” for mass based molecular imaging. SCiLS™ has also partnered withMetaboScape®for precise image annotation from databases or LC/MS runs. This unique combination powers SpatialOMx®to provide important biological context to your bulk or single-cell OMCIS work.
Применения
Combining X-Omics performance with high MALDI sensitivity
timsTOF fleX uniquely enables SpatialOMx®
Whether it’s proteomics, lipidomics, glycomics, or metabolomics, the timsTOF fleX is the ideal platform for deep SpatialOMx®分析。使用专利的SmartBeam 3D技术来进行快速，无标签的MALDI成像，以映射样品中分子的分布，并确定感兴趣的区域以进行更具体的分析。应用Pasef®powered LC-MS/MS for the highest level of identifications and for greatest confidence in your results.
肿瘤不仅仅是眼睛
癌症的微环境包含健康细胞，肿瘤细胞，结缔组织，血管和炎症在不同时间点的组合。这些成分中的每一个都将具有自己独特的化合物分子特征。研究疾病国家的研究人员在很大程度上依赖于解释组织病理学并在生物分子的背景下创建这些地图，从而弥合了传统的OMICS与理解疾病之间的差距。
找到与CCS感知空间X-omics的区别
Cancer cells and other disease states have significant genetic and epigenetic modifications that influence the genomic expression cascade. Whether you are looking at the proteome, lipidome or metabolome, the spatial distribution of compounds contains valuable information for interpretation. Understanding complex samples require unmatched analytical depth provided by the ion mobility option of the timsTOF fleX in addition to mass and charge. Isobaric interferences can be distinguished, and isomers separated. This helps for example to localize isobaric lipids in tissues. In-situ MS/MS as well as PASEF®授权的4D X-Momics为您提供了识别您感兴趣的分析物的能力。
Introducing an automated molecular annotation workflow for SpatialOMx®
Using Bruker’s industry leading software, it is now possible to annotate target molecules directly from tissue. Simply load your data into SCiLS™ Lab, define regions of interest, and export the peak lists to MetaboScape®。使用LC-MS/MS分析的数据库或化合物列表来注释您的峰值，然后查看并将注释导出回SCILS™以进行可视化。从SCILS™中，您可以通过途径和熟悉的命名法而不是质量标签来可视化结果，以减少从数据到结果的时间。
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Отзывы
“The timsTOF fleX is an innovative instrument that synergizes multiple analytical capabilities to allow development of novel omics workflows. For imaging MS, it may be potentially transformative, especially for tissue metabolomics and glycomic applications.”
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Дополнительная информация
Literature Room Mass Spectrometry
Поддержка
Специалист
For Research Use Only. Not for use in clinical diagnostic procedures.