NMR和EPR Pharma Solutions

Biologics Higher Order Structure

评估生理相关缓冲液中完整蛋白/抗体的高阶结构(HOS)。在原子分辨率下检测到HOS的小但相关的变化。

Основные моменты

True HOS
Evaluates all aspects (primary, secondary, tertiary, and quaternary) of HOS.
Atomic Resolution
Small but relevant changes in the HOS can be detected using 1D PROFILE, ECHOS, CCSD, and/or PCA. Each of these methods highlight spectral differences, which can be assigned to a region in the protein.
生理条件
NMR采集方法最近得到了增强,在生理相关条件下,在自然丰度下对完整分子(150 kDa)进行了研究。

最新一代的NMR仪器与新开发的NMR采集方法相结合,意味着完整的分子,包括抗体(150 kDa),可以在自然丰度,配方缓冲液或其他与生理学相关的条件下进行表征。Differences between batches are detected by comparing the spectrum of a batch with a reference, thus allowing rapid pass/fail decisions to be made.

Особенности

特征

Easy acquisition:最新一代的NMR仪器提供了高光谱分辨率和高灵敏度。当与新开发的NMR采集方法结合使用时,这可以研究完整的分子,包括在配方缓冲液或其他生理相关条件下自然丰度的抗体(150 kDa)。

易于分析:易于使用的NMR分析软件还使非NMR专家可以访问此解决方案。

  • Similarity assessment: Published & accepted methods are available
    o PROFILE 1D
    o PCA – unsupervised chemometrics
    建设和distan o多元统计模型ce calculations
    o ECHOS – simple representation of spectral differences
    o CCSD – represents shifts in peak positions.
  • 矩阵分析以比较批次
  • 集成解决方案:链接数据和结果,从而为确定的任何观察到的光谱差异提供了原因。

Click here to view exclusive biologics and biosimilars content

Применения

Программное обеспечение

Software

Mnova BioHOS software features

  • Easy-to-use NMR analysis software makes this solution accessible to non-NMR experts
  • Similarity assessment. Published & accepted methods are available
    - 配置文件1D
    - PCA – unsupervised chemometrics
    - Multivariate statistic model building and distance calculations
    - ECHOS – simple representation of spectral differences
    - CCSD – represents shifts in peak positions
  • 矩阵分析以比较批次
  • Integrated solution: data and results are linked, giving insight on the potential reason(s) for observed spectral differences.

Вебинары

Отзывы

Testimonials

NMR lab visit at Health Canada
2D NMR Methods to Quantify Heparin Composition (Pharmaceutical Analysis
Quality assessment of biologics and biosimilars at atomic level

Dr. Fabio Baroni, Merck KGaA, Rome, Italy

The need for high-resolution techniques for the characterization of Higher Order Structure of biologics is regarded as being extremely urgent, especially in the case of application to biosimilar development.
NMR, besides assuring an atomic-level fingerprint of the molecule’s structure, grants a resolution and sensitivity to structural changes incomparable with any other spectroscopic technique. Bruker, has led multiple developments in NMR during the last decade in terms of improved instrumentation, clear workflows and enhanced data analysis and finally, has made the employment of the technique in biosimilar R&D.

Поддержка

Labscape

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory