Transgenic line expressing His2Av-mCherry as fluorescent nuclear reporter. The fruit fly embryo was imaged for almost one complete day (4 × 200 slices every 30 seconds). Imaged on the MuVi SPIM.
提供:
Lars Hufnagel
欧洲分子生物学实验室(EMBL)
德国海德堡
Cell tracking created with arivis Vision4D 3D visualization and analysis software. Imaged on the MuVi SPIM.
提供:
Celia Smits and Stanislav Y. Shvartsman
分子生物学系
新泽西州普林斯顿大学
USA
斑马鱼在Muvi Spim上成像。从Imaris的5个堆栈缝合,每340片,16小时/10分钟。可以观察到鱼类的生长。
提供:
Jingxia Liu教授
华宗农业大学
中国
Zebrafish embryo expressing Histone H2A-GFP imaged every 6 min from late gastrula to 15–17 somite stage. Imaged on the MuVi SPIM.
提供:
安德烈斯·科拉索(Andres Collazo)
加利福尼亚州帕萨迪纳的加利福尼亚州加利福尼亚州
as well as: Course faculty and participants of the 2017 Zebrafish Course
Marine Biological Laboratory (MBL)
Woods Hole, MA, USA
From left to right: the video shows a beating Zebrafish heart imaged at 50 frames/sec, followed by Zebrafish blood vessels (magenta) and red blood cells (yellow) and Zebrafish blood flow imaged at 50 frames/sec. Imaged on the MuVi SPIM.
提供:
纳迪亚梅&伊内斯品牌
伯尔尼大学
Bern, Switzerland
果蝇卵巢用伪胶蛋白染色,沿膜和生殖线环的标记肌动蛋白(红色),DAPI(蓝色)以显示核,以及一个体细胞和体环渠标记(绿色),以标记上皮中的环管。
提供:
Jasmin Imran Alsous
Schvartsman实验室
Princeton University, Princeton, NJ, USA
hESC-derived胰腺球体。成像在TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)
提供:
Yung Hae Kim
Graphin-Botton Group, MPI-CBG
德累斯顿,德国
Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.
A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066
3D culture system of human primary cells imaged on the QuVi SPIM.
提供:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK
用抗GFAP(Alexa 488)染色的球体标记星形胶质细胞和抗神经丝200(Alexa555),以标记神经元。在Invi Spim上成像。
提供:
Markus Bruell
康斯坦茨大学AG Leist
德国康斯坦茨
在QUVI SPIM上成像的人类原代细胞的3D培养系统(330µm x 220 µm x 1200µm)。成像在Quvi Spim上。
提供:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK
Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.
提供:
Martin Stöckl
康斯坦茨大学
Germany
稳定表达H2B-MCHERRY和IRFP670的小鼠胚胎干细胞的菌落具有膜靶向信号。在Invi Spim上成像。
提供:
皮埃尔·尼维(Pierre Neveu)
欧洲分子生物学实验室(EMBL)
德国海德堡
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
Imaged on the InVi SPIM Lattice Pro.
可视化:Imaris(Bitplane)。
提供:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
德国海德堡
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.
右:表示CENPC-EGFP和H2B-MCHERRY的小鼠卵母细胞进行动力学跟踪。
在Invi Spim上成像。
Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145
对F-肌动蛋白和染色质染色的上皮细胞系(BS-C-1)。使用Invi Spim晶格Pro拍摄的图像。
提供:
Ulrike Engel
尼康成像中心
University of Heidelberg
Germany
表达GFP和MCHERRY的HELA细胞。在Invi Spim上成像。
提供:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands
The axon of a neuron in the zebrafish brain was selectively dissected by means of IR laser ablation (MuVi SPIM). Thirty minutes after ablation, four microglia reached the damaged axon.
Image taken from:
de Medeiros, G., Kromm, D., Balazs, B. et al. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep 10, 1942(2020).https://doi.org/10.1038/s41598-019-54349-x
The histone marker (H2A::mCherry, purple) and the membrane marker (cnd-1::GFP, green) were used for lineage tracing in C. elegans. Imaged on the QuVi SPIM.
提供:
Zhirong Bao
Zhirong Bao实验室
Memorial Sloan Kettering Cancer Center (MSKCC)
美国尼约克
Microglia movement in zebrafish. The vascular system is labeled with a cyan marker and microglia with a yellow one. Imaged on the QuVi SPIM at 2 FPS for 20 min. Two orthogonal views fused and max. project.
提供:
N. Norlin, F. peri
欧洲分子生物学实验室(EMBL)
德国海德堡
Zebrafish eye imaged on the MuVi SPIM.
提供:
Anja Machate and Michael Brand
Center for Regenerative Therapies Dresden (CRTD), TU Dresden
德累斯顿,德国
毛细胞在新生小鼠耳蜗中为GFP染色。以62.5倍的放大倍数成像。在Invi Spim上成像。
提供:
Raphael Etournay
Genetics and Physiology of Hearing, Institut Pasteur
Paris, France
用Muvi Spim成像的转基因拟南芥根表达核包膜标记。视频的比较显示了重力对根生长的影响。
提供:
Shanjin Huang
Tsinghua University
Beijing, China
Transgenic Arabidopsis root expressing a membrane marker. Imaged with the InVi SPIM.
提供:
Alexis Maizel
海德堡大学COS
德国海德堡
微藻中的自发荧光。在Invi Spim上成像。
Mammalian tumors (red) growing in zebrafish (green). Imaged on the MuVi SPIM
提供:
Dr. Xi Yao and Dr. Zhangzhao Junjie
Model Animal Research Center of Nanjing University
中国
Zebrafish heart beating imaged on the MuVi SPIM.
提供:
Jingxia Liu教授
华宗农业大学
中国
Protein dynamics in C. elegans skeleton. Imaged on the InVi SPIM.
提供:
Chai Yongping博士和Ou Guangshuo博士
Tsinghua University
中国
在Muvi Spim上成像的水坝中的自发荧光。
提供:
艾伦·德·德·埃斯特克(Ellen Decaestecker)和卢克·德·梅斯特(Luc de Meester)
KU Leuven
Kortrijk, Belgium