Luxendo Light-Sheet Microscopes

TruLive3D Imager

双面照明灯页显微镜
TruLive3D-Imager-Dual-Sided-Illumination-Light-Sheet-Microscope-Top-Down-Transparent-BRUKER

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TruLive3D Imager

Optimized for fast 3D multi-sample volume imaging of delicate live specimen in their native 3D environment, the TruLive3D Imager is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, organoids, oocytes and more, enabling time-lapse, high-throughput imaging experiments. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects.

深而清晰的成像
Dual-sided illumination and single-lens detection from below for fast acquisition speed, high-resolution imaging, and minimal shadowing effects.
天然和自然
近距离敏感样品的长期成像,具有降低的光毒性作用的长期成像。
Multiplexing
A large sample holder enables analyzing up to 100s of samples in one experiment with up to six different experimental conditions.

características

紧凑,健壮和无振动设计

The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.

该级1激光系统量身定制,以适合您的实验室长凳,因为所有移动组件都是轻巧且平衡的,因此不需要任何空中桌或振动补偿机构。还保证了焦点和热条件的最大稳定性。专有的压电驱动器阶段可确保长寿和精确度,以实现永久准确的标本定位。由于独特且非常柔和的图像收购概念,图像和样品质量都不会受到影响。

深而清晰的成像

照明和检测

The TruLive3D Imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.

该系统从下面具有双面照明和单镜头检测,从而可以快速获取速度,高分辨率成像和最小的阴影效果。广场成像选项有助于样品定位。

两个Nikon CFI计划荧光10x W 0.3 Na浸入物镜es project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.

天然和自然

Trulive3D成像器配备了环境控制。可以在20–37°C之间调节温度,以达到最佳的孵育条件。

In addition, the TruLive3D Imager also provides precise and stable environmental control (i.e. CO2,o2,n2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2,O的1-21%2,H和20%的H2o(湿度)。气体加湿器为精确调节提供了反馈控制。

Trulive3D成像仪已针对其本机3D环境中精致的活标本的快速3D多样本成像进行了优化。

Multiplexing

Large Sample Holder

Trulive3D成像仪中的扩展样品室适合一个大型样品持有人(长度= 75 mm),可容纳数十个样品。它设计用于小胚胎(例如斑马鱼,果蝇或小鼠),3D球体,卵母细胞等的多位成像。

支架衬里的FEP箔具有双重功能,“弯曲的盖玻璃”以及浸入培养基和样品培养基之间的物理屏障。

Trulive3D菜

新的Trulive3D Dish Dish系列一次性样品持有人无缝集成到系统中,并允许在一个实验中测试不同的媒体条件。

新的现成Trulive3D菜肴与Trulive3D成像仪完全兼容。排列三个一次性菜肴的可能性扩大了系统的能力,以使在不同的实验条件下生长的样品的平行成像。

LUX数据 - 全面的数据处理和存储解决方案

尖端的生物成像,例如灯页显微镜,以及超分辨率或两光子成像,它们会产生大量数据,以为研究人员提供有关其样品的所有相关信息。但是,数据的存储,传输和处理仍然是一个挑战。

Aplicaciones

Live Sample Applications

Browse a selection of applications data from our customers below. Researchers are using the TruLive3D Imager in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

胰腺

hESC来源的胰腺球。在Trulive3D成像器上成像可以在一个实验中收集几个样品的信息。可视化:Imaris(Bitplane)

Courtesy of:
Yung Hae Kim
Grapin-Botton Group, MPI-CBG
德累斯顿,德国

Especificaciones

规格

Illumination 检测
Effective Magnification
视场 像素大小 光学分辨率
10x / 0.3 Na 尼康25x / 1.1 NA

31.3x

62.5x

420 µm

210 µm

208 nm

104 nm

255 nm

Illumination Optics

  • 从440到660 nm的色度校正
  • Light-sheet generation by beam scanning
  • 柔性浅层厚度(2 µm至6 µm)
  • 电动ND滤清器集(90%,10%,1%)用于使用低激光功率
  • 两个Nikon CFI计划荧光10x W 0.3 Na浸入物镜

检测Optics

  • 一个Nikon CFI APO 25X W 1.1 NA浸入物镜镜头
  • 两个光谱检测通道,每个频谱检测通道配备了快速滤轮(在相邻位置之间的10个位置和50 ms切换时间),适用于选定的激光线
  • 同时双通道获取的三个电动二色性镜像位置
  • 电动领环控制Two高速SCMOS摄像机Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

Accesorios

配音

Trulive3D菜es

truelive3D成像器样品持有人

Software

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。

电子,显微镜软件和计算机

  • 带有开放通信接口的嵌入式显微镜软件:已记录的API,基于TCP/IP的通信
  • 用于交互式显微镜控制和实验设计的灵活GUI
  • 带有256 GB RAM的计算机,Intel Dual 8 Core CPU
  • 用于数据流的高速RAID控制器,RAID中的8×4 TB本地存储0
  • GeForce RTX 3070图形卡
  • 10 Gbit/s板载以太网端口
  • 1 UHTV 43英寸显示屏

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