Optimized for fast 3D multi-sample volume imaging of delicate live specimen in their native 3D environment, the TruLive3D Imager is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, organoids, oocytes and more, enabling time-lapse, high-throughput imaging experiments. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects.
The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.
该级1激光系统量身定制,以适合您的实验室长凳,因为所有移动组件都是轻巧且平衡的,因此不需要任何空中桌或振动补偿机构。还保证了焦点和热条件的最大稳定性。专有的压电驱动器阶段可确保长寿和精确度,以实现永久准确的标本定位。由于独特且非常柔和的图像收购概念,图像和样品质量都不会受到影响。
The TruLive3D Imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
该系统从下面具有双面照明和单镜头检测,从而可以快速获取速度,高分辨率成像和最小的阴影效果。广场成像选项有助于样品定位。
两个Nikon CFI计划荧光10x W 0.3 Na浸入物镜es project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.
Trulive3D成像器配备了环境控制。可以在20–37°C之间调节温度,以达到最佳的孵育条件。
In addition, the TruLive3D Imager also provides precise and stable environmental control (i.e. CO2,o2,n2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2,O的1-21%2,H和20%的H2o(湿度)。气体加湿器为精确调节提供了反馈控制。
Trulive3D成像仪已针对其本机3D环境中精致的活标本的快速3D多样本成像进行了优化。
Trulive3D成像仪中的扩展样品室适合一个大型样品持有人(长度= 75 mm),可容纳数十个样品。它设计用于小胚胎(例如斑马鱼,果蝇或小鼠),3D球体,卵母细胞等的多位成像。
支架衬里的FEP箔具有双重功能,“弯曲的盖玻璃”以及浸入培养基和样品培养基之间的物理屏障。
新的Trulive3D Dish Dish系列一次性样品持有人无缝集成到系统中,并允许在一个实验中测试不同的媒体条件。
新的现成Trulive3D菜肴与Trulive3D成像仪完全兼容。排列三个一次性菜肴的可能性扩大了系统的能力,以使在不同的实验条件下生长的样品的平行成像。
尖端的生物成像,例如灯页显微镜,以及超分辨率或两光子成像,它们会产生大量数据,以为研究人员提供有关其样品的所有相关信息。但是,数据的存储,传输和处理仍然是一个挑战。
Browse a selection of applications data from our customers below. Researchers are using the TruLive3D Imager in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
hESC来源的胰腺球。在Trulive3D成像器上成像可以在一个实验中收集几个样品的信息。可视化:Imaris(Bitplane)
Courtesy of:
Yung Hae Kim
Grapin-Botton Group, MPI-CBG
德累斯顿,德国
Illumination | 检测 |
Effective Magnification |
视场 | 像素大小 | 光学分辨率 |
10x / 0.3 Na | 尼康25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
Illumination Optics
检测Optics
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。