TruLive3D Imager

The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.

The TruLive3D Imager is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.

我n addition, the TruLive3D Imager also provides precise and stable environmental control (i.e. CO₂, O₂, N₂, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO₂, 1–21 % for O_2,and 20–99 % for H₂O (humidity). The gas humidifier offers feedback control for precise regulation.

The TruLive3D Imager is optimized for fast 3D multi-sample imaging of delicate live specimen in their native 3D environment.

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Browse a selection of applications data from our customers below. Researchers are using the TruLive3D Imager in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

我llumination Optics

• Chromatic correction from 440 to 660 nm
• Light-sheet generation by beam scanning
• Flexible light-sheet thickness (2 µm to 6 µm)
• Motorised ND filter set (90%, 10%, 1%) for high flexibility in using low laser power
• Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens

Detection Optics

• One Nikon CFI Apo 25x W 1.1 NA water immersion objective lens

• Two spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions) Filters adapted to the selected laser lines

• Three motorised dichroic mirror positions for simultaneous dual channel acquisition

• Motorised collar ring controlTwo high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3

• Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping

• Peak quantum efficiency (QE): 82% @ 560 nm
  

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Electronics, Microscope Software and Computer

• Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication

• Flexible GUI for interactive microscope control and experiment design

• Computer with 256 GB RAM, Intel dual 8 core CPU

• High-speed RAID controller for data streaming, 8×4 TB local storage in RAID 0

• GeForce RTX 3070 graphics card

• 10 Gbit/s on-board Ethernet port

• 1 UHTV 43 inch display