Cleared Sample Imaging
Applications
Cleared-Sample / Cleared-Tissue应用程序
The 3D imaging capabilities of light-sheet microscopes, in combination with optical clearing techniques, enable visualization of very large, cleared specimens in-toto, comprehensive and non-destructive within minutes. It has thus become a valuable tool for 3D microstructure analysis of tissues (e.g. in neuroscience, developmental biology and connectomics).
The MuVi SPIM CS and the LCS SPIM are optimized for 3D imaging of intricate tissue structures, relevant to studying the brain or the central nervous tissue in neuroscience, to analyzing organ development or to investigating tumor structure and genesis in oncology. They provide innovative solutions in sample mounting, sample size and optics for best-in-class 3D imaging of cleared samples. While the MuVi SPIM CS enables imaging at subcellular resolution and samples sizes up to whole mouse brains, the LCS SPIM is optimized for very large samples up to entire adult mice realizing visualization of complete neuronal or vasculature networks at cellular resolution.
Cleared Mouse Lymph Node
MuVi SPIM CS
Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on theMuVi SPIM CS.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Cleared Mouse Lymph Node
QuVi SPIM
BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on theQuVi SPIM.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Cleared Mouse Head
Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5).
Imaged on theMuVi SPIM CS.
Courtesy of:
Glenda Comai
Institut Pasteur
Paris, France
转基因果蝇Expressin行g UAS-mCD8-GFP with the Mef2-GAL4 Driver Line
The fruit fly was optically cleared and RI adjusted using alcohol dilutions and ECI. Size: 1.7 mm length (head to tail).
Imaged on theLCS SPIM.
Courtesy of:
Susanne Önel
Philipps University Marburg
Marburg, Germany
Neuronal Network
Neuronal network (stained with GFP) of a CUBIC-cleared mouse brain
Color-coded depth representation: maximal depth displayed 1.23 mm.
Imaged on theMuVi SPIM CS.
Courtesy of:
Montserrat Coll Lladó
European Molecular Biology Laboratory (EMBL)
Barcelona, Spain
Developing Nerves in a Whole Mouse Embryo
Developing nerves in a whole mouse embryo.
Sample cleared with DBE. Tiled image (3 x 4) acquisition. Scalebars: 1mm.
Imaged on theLCS SPIM.
Sample courtesy of:
James Muller
MSKCC
New York, USA
