Cleared Sample Imaging
Applicazioni
Cleared-Sample / Cleared-Tissue Applications
The 3D imaging capabilities of light-sheet microscopes, in combination with optical clearing techniques, enable visualization of very large, cleared specimens in-toto, comprehensive and non-destructive within minutes. It has thus become a valuable tool for 3D microstructure analysis of tissues (e.g. in neuroscience, developmental biology and connectomics).
MUVI SPIM CS和LCS SPIM经过优化，用于3D复杂的组织结构成像，与研究神经科学中的大脑或中枢神经组织有关，分析器官发育或研究肿瘤学中的肿瘤结构和生殖。它们提供了样品安装，样本量和光学元件的创新解决方案，用于清除样品的最佳3D成像。尽管MUVI SPIM CS可以在亚细胞分辨率和样品尺寸上进行成像至整个小鼠大脑，但LCS SPIM对非常大的样品进行了优化，直到整个成年小鼠实现了在细胞分辨率下实现完整神经元或脉管网络的可视化。
Cleared Mouse Lymph Node
muvi spim cs
清除的小鼠淋巴结。高内皮静脉（642 nm，红色）和自动荧光（488 nm，绿色），以可视化周围的组织。成像muvi spim cs.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Cleared Mouse Lymph Node
QuVi SPIM
BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on theQuVi SPIM.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
清除的鼠标头
Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5).
成像muvi spim cs.
Courtesy of:
Glenda Comai
Institut Pasteur
法国巴黎
转基因果蝇Expressin行g UAS-mCD8-GFP with the Mef2-GAL4 Driver Line
将果蝇光学清除，并使用酒精稀释液和ECI调整RI。尺寸：1.7毫米长（头到尾）。
成像LCS SPIM.
Courtesy of:
Susanne Önel
Philipps University Marburg
Marburg, Germany
神经元网络
神经元网络（用GFP染色）的小鼠脑
Color-coded depth representation: maximal depth displayed 1.23 mm.
成像muvi spim cs.
Courtesy of:
Montserrat Coll Lladó
European Molecular Biology Laboratory (EMBL)
Barcelona, Spain
在整个小鼠胚胎中发育神经
Developing nerves in a whole mouse embryo.
用DBE清除样品。瓷砖图像（3 x 4）采集。比例尺：1mm。
成像LCS SPIM.
Sample courtesy of:
James Muller
MSKCC
美国纽约
