muvi spimCS
Punti salienti
Muvi Spim CS启用光学清除样品的3D成像
The sample chamber, the illumination, and the detection unit in the octagon are adapted to match the experimental needs in terms of sample size, refractive index, field-of-view, and resolution, adding high flexibility to the system.
Caratteristiche
清除样品模块
清除的样品模块使MUVI SPIM几何形状适用于大型清除样品成像。可交换的清除样品八角形和安装在显微镜顶板上的架空样品定位单元使其与Muvi Spim的实时样品版本完全兼容。
The octagon can be easily removed from the microscope for maintenance and cleaning of the sample chamber and the objective lenses that have been exposed to the mounting solution. The sample chamber is compatible with a broad range of solutions used in different clearing protocols. Available in two different sizes (max. volumes of 20 ml and 33 ml), the sample chamber can accommodate large samples such as mouse brains and other complete organs. Sample mounting from above enables easy sample access and optimized travel range suited for various mounting methods (e.g. hook, plate, pin or cuvette).
灵活的照明和检测配置利用Muvi Spim CS满足您的应用需求，并允许以高速和高分辨率的各种样品进行成像。
照明和检测
The MuVi SPIM CS can achieve a resolution down to 300nm in 3D (at a wavelength of 500nm), enabling imaging of large optically cleared samples with high resolution.
两个Nikon 10x 0.3 NA空气目标镜头项目来自样品上相对方向的两个对齐光板。检测包括高数值孔径，多层物镜镜片，具有20倍和10倍的放大倍率。另一个放大倍率更换器导致总放大倍数在7.5倍至30倍之间。您可以根据实验需求选择一组照明和检测目标镜头。
muvi spimCS Design
LUX数据 - 全面的数据处理和存储解决方案
尖端的生物成像，例如灯页显微镜，以及超分辨率或两光子成像，它们会产生大量数据，以为研究人员提供有关其样品的所有相关信息。但是，数据的存储，传输和处理仍然是一个挑战。
Applicazioni
Cleared-Sample / Cleared-Tissue Applications
Browse a selection of applications data from our customers below. Researchers are using MuVi SPIM CS in a variety of ways including studies.
清除的小鼠淋巴结
Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on the MuVi SPIM CS.
Courtesy of:
Jens Stein
University of Bern
伯尔尼，瑞士
Mouse Lymph Node
BABB清除的小鼠淋巴结。高内皮静脉（红色）和自动荧光（绿色）以可视化周围的组织。成像在Quvi Spim上。
Courtesy of:
Jens Stein
University of Bern
伯尔尼，瑞士
Neuronal network (stained with GFP) of a CUBIC-cleared mouse brain
颜色编码的深度表示：最大深度显示1.23毫米。
在Muvi Spim CS上成像。
Courtesy of:
蒙特塞拉特·柯尔拉多
European Molecular Biology Laboratory (EMBL)
西班牙巴塞罗那
Cleared Mouse Head
用抗Tuj1（绿色）标记，以标记发育神经和抗抑制剂（红色），以标记分化的肌肉。瓷砖图像（6×5）。在Muvi Spim CS上成像。
Courtesy of:
Glenda Comai
研究所的巴斯德
法国巴黎
Specifiche
规格
| Illumination | 检测 |
Effective Magnification |
视场 | 像素大小 | 光学分辨率 |
| 10x / 0.3 NA | Olympus 20X / 1.0 NA | 15.0x 20.0x 30.0x |
890 µm 665 µm 445 µm |
434 nm 325 nm 217 nm |
300nm |
| 10x / 0.3 NA | Nikon 10x / 0.5 NA | 7.5x 10.0x 15.0x |
1775 µm 1330 µm 890 µm |
867 nm 650 nm 434 nm |
600 nm |
Illumination Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- 柔性浅层厚度（2 µm至8 µm）
- 2 Nikon 10x 0.3 NA air objective lenses
- 2 identical illumination arms
检测Optics
- 可能的不同组合可能：
-1尼康20x 1.0 NA 8 mm WD多污染
- 1 Nikon 10x 0.5 NA 5.5 mm WD multi-immersion - 1 or 2 detection arms, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 1或2高速SCMOS摄像机Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560nm
样品室
样品室大小
| 检测 | Depth (z) | Width (y) | Height (x) |
| 10x | 27mm | 30mm | 40mm |
| 20倍 | 27mm | 30mm | 40mm |
Maximum Imageable Sample Size (ellipsoid shape)
| 检测 | Depth (z) | Width (y) | Height (x) |
| 10x | 11.6mm | 15mm | 20mm |
| 20倍 | 16.4mm | 15mm | 20mm |
Software
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。
电子，显微镜软件和计算机
- 带有开放通信接口的嵌入式显微镜软件：已记录的API，基于TCP/IP的通信
- 用于交互式显微镜控制和实验设计的灵活GUI
- Computer with 256 GB RAM, Intel dual 8 core CPU
- 用于数据流的高速RAID控制器，RAID中的8×4 TB本地存储0
- GeForce RTX 3070图形卡
- 2 x 10 gbit/s，2 x 1 gbit/s，可选2 x光端口
- 43 inch 4k UHD display
