QuVi SPIM
Destaques
QuVi SPIM
The QuVi SPIM is a highly versatile upright, dual view and dual color light-sheet microscope for 3D imaging of living, fixed and cleared samples. Based on our robust, stable system platform, it combines dual-view acquisition with high precision, long travel range stages. The QuVi SPIM is optimized for long-term, high-throughput imaging at subcellular isotropic resolution. It features an easy sample mounting and an intuitive software and hardware concept.
Características
同时Dual-View或双通道采集
Quvi Spim将双重视图与双重检测通道相结合，以实现生命标本和清除样品的大规模3D成像。该系统可以在生活和清除的组织成像之间快速而简单的过渡。
Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.
Optimized Stability
The QuVi SPIM can be equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
In addition, the QuVi SPIM also provides precise and stable environmental control (i.e. CO2，o2，n2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2，O的1-21％2, and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.
User-Friendly Software and Hardware
硬件
它的新型舞台设计允许使用SBS格式板，并通过其快速负载功能提供了简单的样品可访问性。由于客观镜头是可以交换的，因此可以成像各种不同尺寸的样品。
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Many Design Features Make the QuVi Excell
直立，双重视图和双色
The QuVi SPIM combines easy accessibility with high throughput and well-controlled sample conditions, e.g. to perform screening experiments or biophysical methods such as FLIM and FCS.
Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.
照明和检测
Quvi Spim可以在3D中达到340 nm的分辨率，从而实现了实时成像，没有光毒性效应。
The QuVi features a dual-side alternating illumination/detection concept and provides two orthogonal views of the sample.
The illumination optics comprise light-sheet generation by scanning a beam after passing a beam-shaper unit for length and diameter adjustment. Two Nikon 40x 0.8 NA water immersion objective lenses alternatingly project two orthogonal light-sheets on the sample. The expandable and upgradable illumination setup allows a range of up to six lasers. The alignment-free light-source offers 40mW power output for each line. For detection, the same objective lenses are used.
The two spectral detection channels equipped with 10 position high-speed filter wheels and a dichroic mirror changer enable simultaneous dual channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification and speed.
Device Design
The QuVi SPIM's compact, vibration-free and robust design provides maximal stability during your long-term experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behavior of your sample are affected by the gentle image recording.
QuVi SPIM Design
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
aplicações
QuVi Applications
研究人员正在以多种方式使用QUVI SPIM，包括研究胚胎和发育生物学，类器官，细胞培养物，神经生物学和神经发育，植物等。从下面的客户中浏览一系列应用程序数据，或访问我们所有Bruker Lightseet Solutions的广泛画廊。
清除的小鼠淋巴结
BABB清除小鼠淋巴结。高内皮静脉（红色）和自动荧光（绿色）以可视化周围的组织。成像在Quvi Spim上。
Courtesy of:
Jens Stein
University of Bern
伯尔尼，瑞士
3D Cell Culture
3D culture system of human primary cells imaged on the QuVi SPIM.
Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK
C. elegans Lineage Tracing
组蛋白标记(H2A:: mCherry、紫色)和membrane marker (cnd-1::GFP, green) were used for lineage tracing in C. elegans. Imaged on the QuVi SPIM.
Courtesy of:
Zhirong Bao
The Zhirong Bao Lab
纪念斯隆·克特林癌中心（MSKCC）
Ney York, USA
Microglia Movement
Microglia movement in zebrafish. The vascular system is labeled with a cyan marker and microglia with a yellow one. Imaged on the QuVi SPIM at 2 FPS for 20 min. Two orthogonal views fused and max. project.
Courtesy of:
N. Norlin, F. peri
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany
Especificações
规格
| Illumination | Detection |
Effective Magnification |
Field of View | 像素大小 | Optical Resolution |
| 40x / 0.8 NA | 40x / 0.8 NA | 40.0x |
330 µm | 160 nm |
340nm |
Illumination Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- 柔性浅层厚度（2 µm至6 µm）
- Two Nikon CFI Apo 40x W 0.8 NA NIR water immersion objective lenses
Detection Optics
- Two Nikon CFI Apo 40x W 0.8 NA NIR water immersion objective lenses
- 3rd objective lens CFI Plan Achromat 4x 0.1 Air
- Dual-side alternating illumination/detection concept
- 2spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
- 3rd detection path equipped with a Grasshopper3 5.0MP camera for transmitted light detection
Stage
- High-precision XYZ stage
- Unique quick-load feature, SBS plate compatible
- Easy access from above for sample mounting, injections, etc.
- Environmental control unit is optinally available, featuring precise control of temperature (range 20–37°C), humidity, O2和co2
激光组合仪
- Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber)
- 快速调制（MHz范围）和高消光比
Software
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- 用于交互式显微镜控制和实验设计的灵活GUI
- Computer with 128 GB RAM, Intel dual 8 core CPU
- 用于数据流的高速RAID控制器，RAID O中的8×4 TB本地存储
- GeForce RTX 2080 graphics card
- 10 Gbit/s on-board Ethernet port
- 1 UHTV 40 inch display
