maldi-tof/tof

maldi-tof/tof

蛋白质表征,糖蛋白分析,QC应用,聚合物分析,超高吞吐量筛选和MS成像的更聪明的方法
MALDI MS

MALDI-TOF and TOF/TOF MS

The analytical advantages of matrix assisted laser desorption ionization (MALDI) TOF MS have been demonstrated for more than 25 years. With inherent benefits including its speed (time to results) and application flexibility, the convenience and effectiveness of MALDI-TOF MS is magnified by virtue of its low sample volume requirements and broad sample content (salts, buffers) amenability. Dramatic increases in the depth of MALDI analyses have been made routine by intuitive software, and easy automation allows for high throughput solutions.

布鲁克(Bruker)的Flex系列仪器可从市场领先的技术平台提供可靠的结果 - 众所周知,以出色的性能,可靠性,便利性和创新设计。从快速筛选到全面的目标表征,MALDI-TOF MS提供了广泛的实用程序,可满足所有实验室需求。专家用户和初学者可以快速,自信地利用布鲁克的Flex系列MALDI-TOF系统的分析能力和灵活性。

maldi-tof/tof

MALDI-TOF Technology

在过去的25年中,矩阵辅助激光解吸/电离(MALDI)质谱法已演变为具有多种应用范围的功能强大,高度的技术。MALDI耦合到飞行时间质谱法(MALDI-TOF MS)用于对蛋白质进行测序,在组织中绘制生物分子,鉴定微生物,并在一天内分析数千种生化测定。

通过MALDI-TOF MS进行分析的样品是通过与能量吸收矩阵的溶液混合或涂层来制备的,该矩阵在干燥时陷入并批准样品。矩阵用激光束离子化,并将电荷转移到分析物中,从而从样品中的分析物中产生单一充电的离子,然后加速。在使用TOF质量分析仪检测和测量之前,根据其质量与电荷比(M/Z)将离子彼此分开。

与MALDI-TOF MS相关的高吞吐量和速度使该技术成为大规模蛋白质组学,成像研究和其他分析的重要工具。

MALDI-TOF过程
maldi-tof/tofProcess
Bruker的Maldi成像解决方案 - 获得更深入的见解
maldi-tof/tof

MALDI申请

自从80年代中期发现马尔迪(Maldi)以来,这种快速,高通量使电离成为了各种应用。MALDI-TOF分析从样本到结果的固有速度可以快速回答您的分析问题。瞄准不同位置的激光会产生如使用的空间,分子信息。用于组织的MALDI成像。

MALDI成像

For MALDI Imaging, rapid data collection speeds, maintenance of critical spatial resolution, and broad dynamic range are mandatory, and, in conjunction with market-leading SCiLS™ Lab software, are addressed best using ultrafleXtreme or rapifleX® instruments. The unbeaten speed of the rapifleX® enables ultra-high throughput screening and together with our MALDI PharmaPulse 2.2 solution rapid delivery of needed results is ensured. These data are also used to classify tissue – so called tissue typing.

聚合物分析

MALDI-TOF technology offers a unique depth of characterization for many diverse polymer classes. Including bulk material screens, pharmaceutical development, or finished surface analyses, MALDI-TOF MS offers direct molecular weight distributions in fast and versatile workflows.

质量控制

MALDI-TOF MS和MS/MS工作流程在阳性和负离子模式下进行各种可用的电离矩阵以及分析的选项,在肽,寡核苷酸和许多类别的聚合物的质量控制方面具有关键的优势。根据所需的分析深度,Microflex®LRF或AutoFlex®Max系列与Biopharma Cossipne Software结合使用是完美的解决方案。

生物标志物发现

分子变化起源于细胞水平,基于溶液的技术可能缺乏对捕获小细胞群体产生的变化的敏感性,而MALDI可以直接起作用组织。

Proteomics

Protein or peptide characterization using MALDI-TOF MS workflows quickly provide primary protein sequences of intact protein targets in just minutes. Also, higher level protein structure can be examined via the detection and localization of di- and trisulfide bonds. ultrafleXtreme and rapifleX® are the most suited MALDI-TOF instruments to perform top-down, middle-down, and bottom-up sequencing approaches as well as glycoprotein analysis.

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仅用于研究。不用于临床诊断程序。