OT-AFM Combi-System

OT-AFM组合系统配对AFM的特殊表面力测量和成像能力与光学镊子在3D中施加和测量最小力的能力。组合的设置满足了机械稳定性，灵活性和模块化的最高需求。专门设计的OT-AFM ConnectorStage™是将NanoWizard®或Cellhesion®家族的任何AFM与Nanotracker™光学镊子结合在研究级倒置光学显微镜上的关键。

The unique combination of 3D positioning, detection, and manipulation provided by OT and the high-resolution imaging and surface property characterization of AFM opens up a whole new spectrum of applications, such as cellular response, cell-cell or cell matrix interactions, immune response, infection or bacterial/virus/nanoparticle uptake processes, and more.

OT-AFM具有多种可用的手柄，交互和检测位点，可显着扩展单分子应用的范围。

1. DNA发夹解压缩（AFM），而光学陷阱可用于抑制（高激光功率）或量化旋转（低激光功率）。

2. Scanning of a decorated DNA molecule. The molecule with DNA binding proteins (green) is spanned between two optically trapped beads. A functionalized AFM tip (blue) scans along the molecule and whenever interactions between the DNA-attached proteins and the tip occur, these can be detected in the AFM and OT signals.

3. Monitoring of DNA-enzyme (e.g. polymerase, helicase) dynamics. With onestrand attached to an optically trapped particle, the step-wise motion can be tracked. Closed-loop force clamping allows maintaining a constant force on the single strand.

Cellular response, cell-cell or cell matrix interactions, immune response, infection or bacterial/virus/nanoparticle uptake processes are just a few of the examples that can be investigated with JPK’s new state of the art OT-AFM platform. JPKs proven AFM and OT core technologies, combined with fluorescence microscopy, have set the ultimate benchmark for live cell applications.

[1] + [2]: Activation of cells with functionalized beads, parallel AFM measurement. Signaling molecules on the surface of a microparticle are brought in contact with the cell at defined positions and time points.

[5] + [6]: A mechano-sensitive cell is stimulated by a periodic force, exerted by an optically trapped particle. Internal rearrangements of the cytoskeleton alter the mechanical properties of the cell. These properties are easily accessed with AFM methods like force mapping or JPK’s Quantitative Imaging Advanced (QI™-Advanced).

[3] + [4]: AFM can be used in parallel to monitor changes in the cell structure, e.g. by monitoring mechanical properties throughout the process or by molecular recognition force spectroscopy that investigates the distribution and mechanical behavior of membrane proteins.

Trigger cellular responses by using functionalized particles or modified microorganisms is a common method. The resulting changes in cellular structure, dynamics, and mechanical properties can be investigated using AFM-based methods. Delivering objects to specific regions of interest on the cell, however, is very difficult to achieve. OT provides the perfect tool for manipulating the sample and triggering cellular response, at a precise time and location. This significantly improves the throughput, flexibility, and reproducibility of these studies. In this application, the influence of signaling between dendritic cells (DCs) and regulatory T-cells (T_reg) on the adhesion of conventional T-cells (T_conv) to the same DC is quantified by OT-AFM.

[1] Adhesion experiment with dendritic cells (DC) and conventional T-cells (T_conv). The T_convis attached to a tipless cantilever, then approached to the surface-bound DC. The cantilever is pulled up and the adhesion forces are measured. A regulatory T-cell (T_reg) is attached to and removed from the DC with optical tweezers to test its influence on the binding strength. [2]+[3] Measurement setup. The optical trap (red cross) moves the T_regwhile adhesion measurements are performed with a cantilever-attached T_conv. [4] Detachment work measured for the three situations. Treg attachment reduces DC-T_conv互动。t之后_regis removed, the adhesion level is almost restored. Sample courtesy of Yan Shi, University of Calgary/Tsinghua University, Beijing. The original experiment was designed by Yan Shi et al. (publication in print).

• Imaging, positioning, and manipulation experiments from single molecules to living cells
• Measure forces in 2D and 3D, from 500fN to 10nN, on the same sample
• 完全灵活的模块化设计，具有最宽的模式和配件
• Comprehensive integration with optical microscopy techniques such as TIRF or confocal
• All NanoTracker™, NanoWizard® /CellHesion® 200 systems can be combined to become a complete OT-AFM-platform
• The ConnectorStage™ combines the AFM with the tweezers hardware on all major inverted optical microscopes from Zeiss, Nikon, Olympus, and Leica

• All NanoTracker™, NanoWizard®/CellHesion® 200 systems can be combined to become a complete OT-AFM-platform
• ConnectorStage™可以将AFM与Tweezers硬件结合在所有主要的倒置光学显微镜上，来自Zeiss，Olympus，Nikon，Leica

• Two separate controllers drive the two systems independently:
  
  
  • NanoWizard® AFM controller with software
  • NanoTracker™ OT controller with software

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.