Invi Spim家族
Invi Spim产品
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倒视图
The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular structures, and morphological analysis in real-time.
Typical applications of the microscope include dynamic processes in mammalian cell culture applications and even live imaging of intact and living plant models.
高级照明模块
高级照明模块（AIM）是一个模块化扩展，扩展了Invi Spim的功能：在保持系统的易用性和稳定性的同时，它增加了梁形状的可量身定制的，可固定的交互式适应性，以适合高度特定的要求您的样本。
添加各种光束图案的选择 - 经典的静态高斯灯表，扫描的高斯梁，贝塞尔束和光学晶格，甚至定制的形状 - 可提供独特的灵活性，以优化您的生物成像实验。
您选择的是3D高分辨率成像实验的最佳结果 - 大型视野，高速或最佳空间分辨率。
Low Phototoxicity and Photobleaching
Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens.
最大化的光效率和短照明时间可实现长期成像，而无需损害活标本。
环境控制
The InVi SPIM is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
此外，Invi Spim还提供精确稳定的环境控制（即CO2, O2, N2，湿度）。CO的不同组件的气体浓缩范围在0-15％之间2, 1–21 % for O2H和20％的H2O (humidity). The gas humidifier offers feedback control for precise regulation.
Sample Holder
将V形长样样品支架放置在腔室内。它被FEP箔覆盖，允许样品与腔室的物理分离，而不会影响成像特性。它的尺寸减小可确保使用小样品中量。
可以使用不同的策略将样品放在持有人中进行成像。2D细胞可以直接在箔上生长，如“弯曲盖玻璃”中。3D球体或小胚胎可以滴入槽中并通过引力固定，从而使多个样品排列以进行多位成像。
The minimization of required specimen medium further leverages its functionality.
功能
倒置，快速和灵活
Invi SPIM致力于实时成像，可轻松访问样品室，并控制着生存标本的长期3D荧光成像的环境条件。此外，Invi Spim比旋转磁盘或点扫描共聚焦显微镜要快得多。
它非常适合从细胞培养到早期胚胎（例如果蝇和斑马鱼）的各种样品和应用。
Illumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
该系统具有单方面照明和单镜头检测，从而可以快速获取速度，高分辨率成像。广场成像选项有助于样品定位。
One Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs
The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).
检测光学元件可用于快速，高分辨率和非常敏感的成像。配备10个位置高速滤光轮的两个光谱检测通道和电动二角镜更换器同时实现双通道成像。另外，可以顺序获取两个以上的通道。来自Hamamatsu的强大SCMOS ORCA 4.0 V3摄像机非常适合需要高检测效率，定量和速度的实验。
与其他技术（例如共聚焦激光扫描和旋转盘显微镜）相比，灯表显微镜可以采集更高数量的时间点，同时最大程度地减少照相效果。可以实现对动态生物学过程和快速运动跟踪的更深入了解。
Device Design
The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.
为适合您的实验室长凳而定制的该类别1激光系统不需要任何空气桌或振动补偿机构，因为所有移动组件都是轻巧且平衡的。
Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.
应用
附件
Accesories
Trulive3D菜肴
Invi SpimSample Holder for TruLive3D Dish
Invi Spim标准样品持有人
软件
软件
Luxendo的直观用户界面提供了多维实验的简单设置和执行，而实时控制器则由嵌入式控制器处理，以确保与PC的性能波动无关。
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- 带有256 GB RAM的计算机，Intel Dual 8 Core CPU
- High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
- GeForce RTX 3070 graphics card
- 2 x 10 Gbit/s, 2 x 1 Gbit/s, optional 2x optical port on-board Ethernet port
- 43英寸4K UHD显示器