Invi Spim家族
Invi SpimProducts
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Inverted View
The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular structures, and morphological analysis in real-time.
Typical applications of the microscope include dynamic processes in mammalian cell culture applications and even live imaging of intact and living plant models.
高级照明模块
高级照明模块（AIM）是一个模块化扩展，扩展了Invi Spim的功能：在保持系统的易用性和稳定性的同时，它增加了梁形状的可量身定制的，可固定的交互式适应性，以适合高度特定的要求您的样本。
Adding a choice of various beam patterns – classical static Gaussian light-sheets, scanned Gaussian beams, Bessel beams, and optical lattices, or even customized shapes – provides unique flexibility to optimize your bioimaging experiments.
You choose what gives you the best results for your 3D high-resolution imaging experiment – a large field of view, high speed, or optimal spatial resolution.
Low Phototoxicity and Photobleaching
Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens.
最大化的光效率和短照明时间可实现长期成像，而无需损害活标本。
Environmental Control
The InVi SPIM is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
In addition, the InVi SPIM also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2H和20％的H2O (humidity). The gas humidifier offers feedback control for precise regulation.
Sample Holder
The V-shaped, 3 cm long sample holder is placed inside of the chamber. It is covered with FEP foil allowing the physical separation of the sample from the chamber, without influencing imaging properties. Its reduced size ensures the use of small sample medium volume.
Different strategies can be used to place your sample in the holder for imaging. 2D cells can be grown directly on the foil as in a “curved coverglass”. 3D spheroids or small embryos can be dropped into the trough and held by gravitation, allowing multiple samples to be arranged for multi-position imaging.
The minimization of required specimen medium further leverages its functionality.
特長
Inverted, Fast and Flexible
Dedicated to live imaging, the InVi SPIM offers easy access to the sample chamber and control of environmental conditions for long-term 3D fluorescence imaging of living specimen. Furthermore the InVi SPIM is much faster than a spinning disk or point scanning confocal microscope with much less photons.
It is perfectly suited for a great variety of samples and applications ranging from cell culture to early embryos (e.g. Drosophila and Zebrafish).
Illumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
The system features one-sided illumination and single-lens detection, enabling fast acquisition speed, high-resolution imaging. A wide-field imaging option facilitates sample positioning.
One Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs
The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).
The detection optics are optimized for fast, high-resolution, and very sensitive imaging. The two spectral detection channels equipped with 10 position high-speed filter wheels and a motorized dichroic mirror changer enable simultaneous dual-channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification, and speed.
与其他技术（例如共聚焦激光扫描和旋转盘显微镜）相比，灯表显微镜可以采集更高数量的时间点，同时最大程度地减少照相效果。可以实现对动态生物学过程和快速运动跟踪的更深入了解。
Device Design
The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.
为适合您的实验室长凳而定制的该类别1激光系统不需要任何空气桌或振动补偿机构，因为所有移动组件都是轻巧且平衡的。
Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.
アプリケーション
アクセサリー
Accesories
TruLive3D Dishes
Not available in Germany for InVi SPIM product family.
Invi SpimSample Holder for TruLive3D Dish
Not available in Germany.
Invi SpimStandard Sample Holder
Not available in Germany.
ソフトウェア
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- 带有256 GB RAM的计算机，Intel Dual 8 Core CPU
- High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
- GeForce RTX 3070 graphics card
- 2 x 10 Gbit/s, 2 x 1 Gbit/s, optional 2x optical port on-board Ethernet port
- 43英寸4K UHD显示器