Surface plasmon resonance (SPR)

Sierra SPR®-24 Pro

行业领先的SPR系统提供从测定开发到初始筛选的最高性能

High throughput

八个频道/三个位置的数组启用了24小时内的8800互动

Sierra-SPR-24-Pro

하이라이트

Surface plasmon resonance (SPR)

Tailored for high-content applications

灵活性
Choose any combination from 1 to 8 needles for optimum performance, flexibility and range of applications.
Robust
Designed to perform all SPR applications and capable of handling crude samples such as cell lysates.
24个传感器点
Simultaneous analysis of up to 8 samples injected over 24 sensor spots.
生产率
The 8x3 channel/spot array allows multiple controls or targets on a single sensor chip for maximum read out per single sample injection.

특징

Sierra SPR®-24 Pro

分析生物传感器,用于实时的,无标签的分子相互作用

Continuous Flow SPR Analysis
The SPR-24 Pro system is a continuous flow biosensor, delivering sample and reagents to the detection sensors as continuously flowing streams. When sample or reagent injection stops, a “continuous flow buffer” is passed over the sensors.

SPR分析中连续流缓冲区的配方可能会对要测量的分子相互作用产生重大影响。在许多应用中,需要在各种缓冲环境中测量目标相互作用。

Sierra SPR®-24 Pro

框架注入

Sierra SPR中的框架注入功能®-24 Pro系统允许研究依赖性结合 /作用模式研究。该功能仅需要每种缓冲条件的一小部分,该条件在使用时替换系统缓冲区。应研究的情况下发生的预交换以及解离。解离端后,更换帧溶液缓冲液,系统缓冲区再次流过微流体系统。因此,实验时间和试剂成本保持较低。可以将相同的缓冲液定向到所有流动池,也可以同时测定多达八个不同的缓冲液。

框架注入allows the investigation of up to eight buffer conditions in parallel.
Sierra SPR®-24 Pro

Maximising performance with individual needle control

Individual Needle Control
Sierra SPR中的单个针控(INC)功能®-24 Pro系统允许独立操作八个样品拾音针头。研究人员可以灵活地选择1-8针的任何组合来设计其最佳测定法。

Individual needle control maximizes the performance, flexibility, and scope of applications with the Sierra SPR®-24 Pro, while also saving time, materials, and resources when throughput is not the main requirement.

The eight-needle probe head can simultaneously process 8 sample or reagent solutions. Using the INC feature, users can choose to use any number and/or combination of needles at any time during analysis.

혜택

Sierra SPR®-24 Pro

Throughput on demand

Sierra Spr®-24 Pro delivers faster throughput across a wide range of applications, from kinetic assays with a different target on each sensor to detailed analysis employing multiple in-line controls.

The system uses Bruker’s Hydrodynamic Isolation (HI) continuous flow microfluidic technology to address samples as flowing streams onto the 24 spot SPR+传感器阵列。与可选板处理机器人集成时,Sierra SPR®-24 Pro instrument can assay more than 4400 samples per day, generating more than 8800 control subtracted binding responses.

Sierra SPR®-24 Pro可以轻松地通过API集成到自动调度软件中。

Sierra SPR®-24 Pro

Throughput with performance

The flexible Sierra SPR®-24 Pro系统是高通量应用程序的理想选择。它提供24个检测点,由8个注射针来解决。单个针控(INC)使研究人员可以选择选择1到8针的任何组合,以优化应用程序的性能,灵活性和范围。

24传感器检测阵列在8个流动电池中布置,每个流动池包含3个传感器。可以同时分析多达8个样品,每个分析周期最多生成24个结合测量。所有24个传感器也可以完全独立于其他传感器,从而提供最大的测定设计灵活性。

The versatile system can also save time and materials in the lab, particularly if throughput is not the main requirement.

使用框架注入功能,可以在仪器中同时使用多达八个缓冲区。在单个测定中评估不同pH值,离子强度,清洁剂或溶剂浓度以及副因素或抑制剂的效果的能力会导致更简单,优化的测定最终提高吞吐量和生产率。每个通道设计的四传感器设计为控制分析提供了最大的灵活性,同时为最复杂的分析保持吞吐量。

Sierra SPR®-24 Pro

提高生产率

The design of Sierra SPR®-24 PRO最大化控制分析的灵活性,为最复杂的分析提供了高通量。可以在两个控件和活动传感器上同时分析样品。

对非特异性结合与基质蛋白(如HSA或BSA)的早期分析可能至关重要。额外的在线控制不仅将所需的测定循环的数量减半,还可以通过消除多个样品制剂之间的潜在变化来提高数据质量。

From initial assay development to pre-defined method templates, the Sierra SPR控制软件为每种用例提供​​了完美的解决方案。它将硬件的灵活性与易于使用的拖放方法编辑器结合在一起。定义明确的板和方法库支持个人工作流程结构,数据视图允许对流动池进行实时视觉监视。

소모품

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추가정보

Sierra SPR®-24 PRO

表面等离子体共振的应用


Investigation of condition-dependent binding using frame inject

The traditional investigation of condition-dependent binding demands high reagent costs as well as lengthy run time. Frame inject delivers cost and time savings at low sample consumption.


如何使用胺耦合影响配体密度

找到理想的固定水平以获得最佳的动力学测量至关重要。在这里,我们检查了不同示例目标上的影响参数。


Screening and characterization of small molecule binding to protein targets

通过SPR检测的实时,无标签(RT-LF)分析是小分子药物和候选药物的生物物理表征的强大工具。


筛选和表征生物治疗学

通过SPR检测的实时无标签(RT-LF)分析是蛋白质疗法生物物理表征的强大工具。