LCS SPIM
强调
LCS SPIM
LCS SPIM紧凑的纸张显微镜designed for very fast and very gentle 3D imaging of large optically cleared samples. Like the Luxendo MuVi SPIM CS, it combines the advantages of light-sheet fluorescence microscopy and of tissue clearing methods. Novel and unique solutions facilitate mounting and fast microscopy of much larger samples, rendering it a unique tool in its field. Typical applications of the microscope include imaging of large cleared-tissue samples, e.g. whole mouse brains to visualize complete neuronal and vascular networks.清除器官或厚组织样品的显微镜在发育生物学研究和癌症研究中高度相关，例如研究器官发育，肿瘤结构和肿瘤发生时。
eigenschaften
Compact, Benchtop Design
The LCS SPIM's compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.
The basic configuration of the LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.
该级1激光系统量身定制，以适合您的实验室长凳，因为所有移动组件都是轻巧且平衡的，因此不需要任何空中桌或振动补偿机构。还保证了焦点和热条件的最大稳定性。专有的压电驱动器阶段可确保长寿和精确度，以实现永久准确的标本定位。由于独特且非常温和的图像收购概念，图像和样本质量都不会受到影响。
大而封闭的比色杯
An exchangeable quartz-crystal cuvette simplifies the whole sample mounting procedure as well as cleaning and maintenance, while ensuring compatibility with all clearing solutions by covering a refractive-index (RI) range from 1.33 to 1.57.
该比色绿色有不同的长度和尺寸可适应不同程度的样品：比色杯的尺寸范围从[20 mm×20 mm×20 mm]到[60 mm x 30 mm x 30 mm]（L×W 30 mm x 30 mm）（L×W×H）。
High-Throughput Capability
石英晶体比色杯促进了大型细腻样品的快速安装。简单地将样品引入装有清除溶液的比色杯中。
The motorized sample stage for positioning and stack acquisition as well as the programmable optics concept for fast 3D scanning of the light-sheet through the sample ensure that the sample remains unperturbed during the imaging experiment while achieving the highest acquisition speed.
照明和检测
照明光学元件通过在通过梁形单元后扫描梁以进行长度和直径调节，包括灯页面生成。两个Nikon 4x 0.2 NA空气目标镜头项目来自样品上相对方向的两个对齐光板。可扩展且可升级的照明设置可以非常灵活地选择激光器。
The detection optics comprises one Olympus 4x 0.28NA air/dry lens. An additional magnification changer results in a total magnification between 2.2x and 8.8x. The spatial detection arm is equipped with a 10 position high-speed filter wheel. The robust sCMOS camera is well-suited for experiments that require high detection efficiency, quantification and speed.
LSC SPIM设计
LUX数据 - 全面的数据处理和存储解决方案
Cutting-edge biological imaging such as light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Anwendungen
Cleared-Sample / Cleared-Tissue Applications
Browse a selection of applications data from our customers below. Researchers are using LCS SPIM in a variety of ways including studies.
Spezifikationen
规格
| Illumination | 检测 |
Effective Magnification |
视场 | 像素大小 | 光学分辨率 |
| 4x / 0.2 NA | Olympus 4.0x / 0.28 NA | 2.2x 4.4x 8.8倍 |
6600 µm 3300 µm 1515 µm |
2.96 µm 1.48 µm 0.74 µm |
1.1 µm |
Illumination
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- 柔性灯页厚度
- 2 Nikon 4x 0.2 NA空气目标镜头
- 2 identical illumination arms
- 可选：扩展FOV的轴向光束扫描
检测
- 一个Olympus 4x 0.28 NA空气/干燥物镜镜头
- 一个装有快速滤轮的检测臂（在相邻位置之间的10个位置和50 ms的切换时间）
- Filters adapted to the selected laser lines
- One high-speed sCMOS camera
- Maximum frame rate up to 80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
- 速度：整个小鼠大脑（12 mm x 12 mm x 12 mm）〜5分钟
- 70s per stack
- 4 stacks
- 采样大小：3μm×3μm
快速成像模式
LCS SPIM的基本配置可以通过轴向照明扫描来升级以扩展视场（FOV）并减少阴影效果。
激光组合仪
- Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber), more options available upon request
- 快速调制（MHz范围）和高消光比
Software
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。
电子，显微镜软件和计算机
- 带有开放通信接口的嵌入式显微镜软件：已记录的API，基于TCP/IP的通信
- 用于交互式显微镜控制和实验设计的灵活GUI
- Computer with 256 GB RAM, Intel dual 8 core CPU
- 用于数据流的高速RAID控制器，RAID O中的8×4 TB本地存储
- GeForce RTX 3070图形卡
- 2x10 Gbit/s，2x1 Gbit/s，可选的2x光端口
- 1 UHTV 40 inch display
