The LCS SPIM is a compact light-sheet microscope designed for very fast and very gentle 3D imaging of large optically cleared samples. Like the Luxendo MuVi SPIM CS, it combines the advantages of light-sheet fluorescence microscopy and of tissue clearing methods. Novel and unique solutions facilitate mounting and fast microscopy of much larger samples, rendering it a unique tool in its field. Typical applications of the microscope include imaging of large cleared-tissue samples, e.g. whole mouse brains to visualize complete neuronal and vascular networks.清除器官或厚的组织样品的显微镜在发育生物学研究和癌症研究中高度相关,例如研究器官发育,肿瘤结构和肿瘤发生时。
The LCS SPIM's compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.
The basic configuration of the LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.
为适合您的实验室长凳而定制的该类别1激光系统不需要任何空气桌或振动补偿机构,因为所有移动组件都是轻巧且平衡的。还保证了焦点和热条件的最大稳定性。专有的压电驱动器阶段可确保长寿和精确度,以实现永久准确的标本定位。由于独特且非常柔和的图像获取概念,图像和样品的质量都不会受到影响。
可交换的石英 - 晶体比色绿色简化了整个样品安装程序以及清洁和维护,同时通过覆盖1.33至1.57的折射率(RI)来确保与所有清除溶液的兼容性。
该比色绿色有不同的长度和尺寸可适应不同程度的样品:比色杯的尺寸范围从[20 mm×20 mm×20 mm]到[60 mm x 30 mm x 30 mm](L×W 30 mm x 30 mm)(L×W×H)。
石英晶体比色杯促进了大而精致的样品的快速安装。简单地将样品引入装满清除溶液的比色杯中。
定位和sta的机动样品阶段ck acquisition as well as the programmable optics concept for fast 3D scanning of the light-sheet through the sample ensure that the sample remains unperturbed during the imaging experiment while achieving the highest acquisition speed.
照明光学元件包括通过横梁照射单元后扫描梁以进行长度和直径调节,包括光片生成。两个Nikon 4x 0.2 NA空气目标镜头项目来自样品上相对方向的两个对齐光板。可扩展且可升级的照明设置可以非常灵活地选择激光器。
检测光学元件包括一个Olympus 4x 0.28NA空气/干镜。另一个放大倍率更换器导致2.2倍至8.8倍的总放大倍率。空间检测臂配备了10个位置高速滤轮。强大的SCMOS摄像头非常适合需要高检测效率,定量和速度的实验。
尖端的生物成像,例如灯页显微镜,以及超分辨率或两光子成像,会产生大量数据,以为研究人员提供有关其样品的所有相关信息。但是,数据的存储,传输和处理仍然是一个挑战。
Browse a selection of applications data from our customers below. Researchers are using LCS SPIM in a variety of ways including studies.
Illumination | 检测 |
Effective Magnification |
视场 | 像素大小 | 光学分辨率 |
4x / 0.2 Na | Olympus 4.0x / 0.28 NA | 2.2x 4.4x 8.8倍 |
6600 µm 3300 µm 1515 µm |
2.96 µm 1.48 µm 0.74 µm |
1.1 µm |
Illumination
检测
快速成像模式
LCS SPIM的基本配置可以通过轴向照明扫描来升级以扩展视场(FOV)并减少阴影效果。
激光组合器
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
所有连接的设备的精确定时控制是可靠的实验结果的先决条件。全面控制数据流到存储以及GPU支持的图像处理进一步补充了整体性能。