LCS SPIM
Основные моменты
LCS SPIM
LCS SPIM是一种紧凑的浅色显微镜，设计用于大型光学清除样品的非常快速且非常温和的3D成像。像Luxendo Muvi Spim CS一样，它结合了灯场荧光显微镜和组织清除方法的优势。新颖而独特的解决方案有助于安装和快速显微镜，使其成为其领域中独特的工具。显微镜的典型应用包括大型清除组织样品的成像，例如全小鼠大脑可视化完整的神经元和血管网络。Microscopy of cleared organs or thick tissue samples is highly relevant in developmental biology studies and for cancer research, e.g. when investigating organ development, tumor structure and tumorigenesis.
Особенности
Compact, Benchtop Design
LCS SPIM的紧凑，健壮和无振动的设计即使在长期高通量实验中也可以提供最大稳定性。
LCS的基本配置为清除样品成像提供了具有成本效益的解决方案。基于非常灵活的布局，高度灵活的升级选项扩展了系统的性能，以提高速度和光学性能。
量身定做适合您的实验室板凳,这类1激光system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample are affected thanks to the unique and very gentle image-acquisition concept.
Large and Closed Cuvette
An exchangeable quartz-crystal cuvette simplifies the whole sample mounting procedure as well as cleaning and maintenance, while ensuring compatibility with all clearing solutions by covering a refractive-index (RI) range from 1.33 to 1.57.
The cuvette is available in different lengths and sizes to adapt for samples of different extent: the dimensions of the cuvette range from [20 mm × 20 mm × 20 mm] up to [60 mm x 30 mm x 30 mm] (l × w × h).
High-Throughput Capability
A quartz crystal cuvette facilitates the fast mounting of large, delicate samples. The specimen is simply introduced into the cuvette filled with the clearing solution.
用于定位和堆栈采集的电动样品阶段以及通过样品快速3D扫描的可编程光学概念可确保在成像实验中保持样品在达到最高获取速度的同时保持不受干扰。
照明and Detection
The illumination optics comprise light-sheet generation by scanning a beam after passing a beam-shaper unit for length and diameter adjustment. Two Nikon 4x 0.2 NA air objective lenses project two aligned light sheets from opposing directions on the sample. The expandable and upgradable illumination setup allows a very flexible choice of lasers.
The detection optics comprises one Olympus 4x 0.28NA air/dry lens. An additional magnification changer results in a total magnification between 2.2x and 8.8x. The spatial detection arm is equipped with a 10 position high-speed filter wheel. The robust sCMOS camera is well-suited for experiments that require high detection efficiency, quantification and speed.
LSC SPIM Design
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging such as light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Применения
Cleared-Sample / Cleared-Tissue Applications
从下面的客户浏览一些应用程序数据。研究人员以多种方式使用LCS SPIM，包括研究。
Спецификации
Specifications
| 照明 | Detection |
Effective Magnification |
Field of View | Pixel Size | Optical Resolution |
| 4x / 0.2 NA | Olympus 4.0x / 0.28 Na | 2.2x 4.4x 8.8x |
6600 µm 3300 µm 1515 µm |
2.96 µm 1.48µm 0.74 µm |
1.1 µm |
照明
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- Flexible light-sheet thickness
- 2 Nikon 4x 0.2 NA air objective lenses
- 2 identical illumination arms
- Optionally: axial beam scanning for an extended FOV
Detection
- One Olympus 4x 0.28 NA air/dry objective lens
- One detection arm equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- 适应选定激光线的过滤器
- One high-speed sCMOS camera
- Maximum frame rate up to 80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
- Speed: ~5 min for a whole mouse brain (12 mm x 12 mm x 12 mm)
- 每堆70秒
- 4 stacks
- Sampling size: 3 μm × 3 μm
Fast Imaging Mode
The basic configuration of the LCS SPIM can be upgraded with axial illumination scanning to extend the field of view (FOV) and to reduce shadowing effects.
Laser Combiner
- 最多6拉斯维加斯er wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber), more options available upon request
- Fast modulation (MHz range) and high extinction ratio
Программное обеспечение
软件
Luxendo的直观用户界面提供了多维实验的简单设置和执行，而实时控制器则由嵌入式控制器处理，以确保与PC的性能波动无关。
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- Flexible GUI for interactive microscope control and experiment design
- Computer with 256 GB RAM, Intel dual 8 core CPU
- High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
- GeForce RTX 3070 graphics card
- 2x10 Gbit/s, 2x1 Gbit/s, optional 2x optical port
- 1 UHTV 40英寸显示屏
