LCS Spim
Najważniejsze informacje
LCS Spim
LCS SPIM紧凑的纸张显微镜designed for very fast and very gentle 3D imaging of large optically cleared samples. Like the Luxendo MuVi SPIM CS, it combines the advantages of light-sheet fluorescence microscopy and of tissue clearing methods. Novel and unique solutions facilitate mounting and fast microscopy of much larger samples, rendering it a unique tool in its field. Typical applications of the microscope include imaging of large cleared-tissue samples, e.g. whole mouse brains to visualize complete neuronal and vascular networks.清除器官或厚的组织样品的显微镜在发育生物学研究和癌症研究中高度相关，例如研究器官发育，肿瘤结构和肿瘤发生时。
Cechy charakterystyczne
紧凑，台式设计
The LCS SPIM's compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.
The basic configuration of the LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.
量身定做适合您的实验室板凳,这类1激光system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample are affected thanks to the unique and very gentle image-acquisition concept.
Large and Closed Cuvette
An exchangeable quartz-crystal cuvette simplifies the whole sample mounting procedure as well as cleaning and maintenance, while ensuring compatibility with all clearing solutions by covering a refractive-index (RI) range from 1.33 to 1.57.
The cuvette is available in different lengths and sizes to adapt for samples of different extent: the dimensions of the cuvette range from [20 mm × 20 mm × 20 mm] up to [60 mm x 30 mm x 30 mm] (l × w × h).
High-Throughput Capability
A quartz crystal cuvette facilitates the fast mounting of large, delicate samples. The specimen is simply introduced into the cuvette filled with the clearing solution.
用于定位和堆栈采集的电动样品阶段以及通过样品快速3D扫描的可编程光学概念可确保在成像实验中保持样品在达到最高获取速度的同时保持不受干扰。
照明和检测
照明光学元件包括通过横梁照射单元后扫描梁以进行长度和直径调节，包括光片生成。两个Nikon 4x 0.2 NA空气目标镜头项目来自样品上相对方向的两个对齐光板。可扩展且可升级的照明设置可以非常灵活地选择激光器。
The detection optics comprises one Olympus 4x 0.28NA air/dry lens. An additional magnification changer results in a total magnification between 2.2x and 8.8x. The spatial detection arm is equipped with a 10 position high-speed filter wheel. The robust sCMOS camera is well-suited for experiments that require high detection efficiency, quantification and speed.
LSC SPIM设计
Lux DATA - A Comprehensive Data Processing and Storage Solution
Cutting-edge biological imaging such as light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Aplikacje
清除样本 /清除组织应用程序
Browse a selection of applications data from our customers below. Researchers are using LCS SPIM in a variety of ways including studies.
Specyfikacje
规格
| Illumination | Detection |
有效放大 |
视场 | 像素大小 | Optical Resolution |
| 4x / 0.2 NA | Olympus 4.0x / 0.28 NA | 2.2x 4.4倍 8.8倍 |
6600 µm 3300 µm 1515 µm |
2.96 µm 1.48 µm 0.74 µm |
1.1 µm |
Illumination
- Chromatic correction from 440 to 660 nm
- 光束扫描的灯页生成
- 柔性灯页厚度
- 2 Nikon 4x 0.2 NA air objective lenses
- 2相同的照明臂
- 可选：扩展FOV的轴向光束扫描
Detection
- One Olympus 4x 0.28 NA air/dry objective lens
- One detection arm equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 一台高速SCMOS摄像机
- Maximum frame rate up to 80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- 峰量效率（QE）：82％ @ 560 nm
- Speed: ~5 min for a whole mouse brain (12 mm x 12 mm x 12 mm)
- 70s per stack
- 4 stacks
- Sampling size: 3 μm × 3 μm
快速成像模式
LCS SPIM的基本配置可以通过轴向照明扫描来升级以扩展视场（FOV）并减少阴影效果。
激光组合器
- Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber), more options available upon request
- 快速调制（MHz范围）和高消光比
Oprogragonwanie
Software
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.
Electronics, Microscope Software and Computer
- Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
- 用于交互式显微镜控制和实验设计的灵活GUI
- 带有256 GB RAM的计算机，Intel Dual 8 Core CPU
- 用于数据流的高速RAID控制器，RAID O中的8×4 TB本地存储
- GeForce RTX 3070 graphics card
- 2x10 Gbit/s, 2x1 Gbit/s, optional 2x optical port
- 1 UHTV 40 inch display
