QTOF质谱法
Since its first commercial launch 25 years ago, quadrupole time-of-flight (QTOF) mass spectrometry (MS) has revolutionized numerous fields, ranging from biological and pharmaceutical research to clinical toxicology. The unique hybridization of quadrupole and TOF technology resulted in high-resolution, high mass accuracy analysis of all ions simultaneously, which is today used to identify and quantify complex mixtures or unknown compounds.
The MS/MS capabilities of QTOF MS systems enable coupling to both LC and GC systems and Bruker’s expertise provides a variety of innovative instruments. Our powerful, high performance products are specifically designed to meet the rapidly growing needs of customers in the academic, pharmaceutical, industrial, clinical, and applied markets.
QTOF技术
布鲁克（Bruker）提供了广泛的不同电离源，以允许各种分离技术与高分辨率质谱的好处耦合。在质谱中，MS系统的性能在质量分辨率，质量准确性和主要敏感性方面高度依赖于离子产生方法。
产生离子后，它们将由毛细管入口系统和双离子漏斗引导到四极系统中进行隔离（MS）和受控片段（MS/MS）。
Orthogonal acceleration follows the quadrupolar units and divides the permanent stream of ions in discrete packages being shot into the flight tube. At its top end they get reflected for improved resolving power and focusing onto the detector multi-channel plate.
QTOF Applications
结构蛋白质组学
（BIO）药物科学内部的研究人员越来越多地承担着对效果相互作用和压力源（例如pH或温度）的更深入了解和理解，对生物治疗蛋白的构象结构具有。这是一项非常具有挑战性的任务，研究人员经常使用各种分析工具来研究生物治疗剂的高阶结构，监测蛋白质/蛋白质 - 药物相互作用并执行表位映射。
In recent years Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has emerged as a valuable technique within the arsenal of analytical tools available for providing such insights into protein structure conformation. However, until recently HDX-MS was confined mostly to academic research laboratories, due to a lack of automation and limited data interpretation tools, as well as the need to have reliable monoisotopic mass assignments.
Metabolomics
几个关键步骤对于自信地识别与动态代谢过程相关的化合物很重要。包括样本准备，数据采集和数据评估。
Meaningful insights in a biological context can only be obtained if all of the steps in a workflow are controlled.
The acquisition of high quality LC-MS/MS data follows sample preparation in non-targeted metabolomics workflows, with the T-ReX®LC-QTOF solution, no LC-MS/MS parameter optimization is required. To analyze large sample cohorts which require high retention time stability the Elute UHPLC in combination with the dedicated T-ReX®Elute Metabolomics-kit: RP is provided. The Reversed-Phase LC column kit enables matching of retention times to values in the Bruker HMDB Metabolite Library. The impact II for MS/MS data acquisition uses optimized parameters and its robust performance is the basis for high quality data acquisition, enabling extensive profiling studies of complex samples.
污染物和残留物筛查
Analysing complex food or water samples for pesticides, mycotoxins etc. or equally challenging human or animal urine analysis samples for poisons or drugs is a demanding application. Bruker offers a powerful solution based upon accurate mass analysis and highly curated databases (over 3000 compounds) to ensure reporting false positives (or false negatives) is minimized.
QTOF Related Information
Structural proteomics:
污染物和残留物筛查：
Literature Room Mass Spectrometry
仅用于研究。不用于临床诊断程序。