Timstof Flex

匹配的双源设计PASEF^®具有无标签的空间定位的授权LC-MS/MS识别以解码样品的分子组成

Timstof Flex建立在shot弹枪蛋白质组学标准上smartbeam3D激光光学用于快速测量的一个平台。双源仪器的理想选择^®, timsTOF fleX conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. No single instrument has before provided access to both essential capabilities for the most advanced OMICS researchers.

During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds. No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class OMICS identification and quantification workflows to creating high definition molecular maps of tissue sections to see what matters most.

由于Maldi和ESI生产的离子从源从探测器传播到探测器的相同路径，因此MALDI工作流可以利用Timstof Pro中发现的最先进的特征，包括基于碰撞横截面（CCS）的离子移动性分离（TIMS）（TIMS）（CCS））检测到的分子。可以在ESI模式下进行调整和校准，并用于MALDI实验，以额外优化。

TIMS allows for the separation of molecules dependent on the shape of the ions. Ions are entering the dual TIMS device together with a gas stream and are accumulated in the first section by an electrical field. The actual separation takes place in the second part of the TIMS cartridge. Ions are eluted in a temporal and spatial fashion by lowering the electrical potential. Variable ramp speed and mobility range adaptation gives rise to optimizations for different classes of molecules allowing high flexibility for the user.

It has never been so easy to combine the regiospecificity of MALDI Imaging with in-depth X-Omics analyses. Applicable for examining a wide range of analytes, including metabolites, lipids or glycans, and seamlessly compatible with microscopy workflows, MALDI Imaging can identify regionally specific compound distributions for SpatialOMx^®分析。Cutting-edge instrumentation with a dual-source design paired with proofed high purity consumables for robust analyses and user-friendly software saves time and makes your research life easier. Use Bruker’sIntelliSlidesfor automated MALDI Imaging and SpatialOMx^®workflows on the timsTOF fleX.

The timsTOF fleX is a versatile instrument for probing the molecular content of your sample. Built on Bruker’s pioneering timsTOF Pro platform, the timsTOF fleX is a fully functional high speed, high sensitivity ESI instrument for all your X-Omics analyses, combined with a high spatial resolution MALDI source and stage specifically designed for resolving molecular distributions and bringing a spatial dimension to OMICS analyses. Transform proteomics analysis into spatial proteomics,脂质组学进入空间脂质组学，并代谢组s into spatial metabolomics and get tissue context to your data. Interact with other disciplines and get more out of your analytical data to be successful.

成像初学者的“易用性”工作流程
我们现成的消耗品和软件产品使您能够获取和解释分子组织图像，例如经验丰富的专业人员。自动成像运行基于我们的高级Intellislides™save time and effort and ensure precise results. Reproducible outcomes are guaranteed with our pureFlexMatrix™pre-measured瓶准备提供的涂层。Get access to these consumables and more in Bruker’s webshop.

将原始数据转换为统计处理的信息，并通过SCiLS™ Lab, the “gold standard” for mass based molecular imaging. SCiLS™ has also partnered withMetaboScape^®for precise image annotation from databases or LC/MS runs. This unique combination powers SpatialOMx^®to provide important biological context to your bulk or single-cell OMCIS work.

Timstof Flexuniquely enables SpatialOMx^®
无论是蛋白质组学，脂质组学，甘露科学还是代谢组学，Timstof Flex都是Deep Patialomx的理想平台^®分析。使用专利smartbeam 3 d技术conduct fast, label-free MALDI Imaging for mapping the distribution of molecules in your sample and identify regions of interest for more specific analyses. Apply PASEF^®powered LC-MS/MS for the highest level of identifications and for greatest confidence in your results.

A tumor is more than meets the eye
The microenvironment of cancers contains a combination of healthy cells, tumor cells, connective tissue, blood vessels, and inflammation in different ratios at different time points. Each of these components will have their own unique molecular signature of compounds. Researchers who study disease states rely heavily on interpreting tissue pathology and creating these maps within the context of biomolecules bridges the gap between traditional OMICS and understanding disease.